The mice were perfused with saline to flush out circulating blood cells under anesthesia. Subsequently, the colon tissues were collected, and the lengths were measured in a relaxed position without stretching. Then, the colon tissues were fixed in 10% buffered formaldehyde and embedded in paraffin. The colon tissues were cut and stained with hematoxylin and eosin (HE). HE-stained tissue sections were scanned by a NanoZoomer-XR Scanner C12000 (Hamamatsu Inc., JP) or imaged on an Olympus BX51 (Olympus, JP) microscope using the Spot Insight image capture system CCD camera.
Nanozoomer xr scanner c12000
Manufactured by Hamamatsu Photonics
Sourced in Japan
The NanoZoomer-XR Scanner C12000 is a high-resolution digital slide scanner designed for scanning and digitizing histological samples. It utilizes a high-speed camera and advanced optical technology to capture detailed images of microscopic specimens.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nanozoomer xr scanner c12000
1
Colon Tissue Preparation and Histological Analysis
2
Histological Analysis of Mouse Colon
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The mice were anesthetized with 1% sodium pentobarbital by intraperitoneal injection and sacrificed. Then, the colons were isolated and sectioned. The colons were washed twice in pre-cooled PBS and soaked overnight in paraformaldehyde (4% in 0.1 mol/L phosphate buffer, pH 7.4). After immersion in absolute ethyl alcohol through a graded alcohol series, the tissues were embedded in paraffin, sectioned, and stained. Then, the colonic tissue sections were scanned by a Nanozoomer-XR Scanner C12000 (Hamamatsu Inc., Japan).
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