Hydraulic micropositioner

Manufactured by Kopf Instruments

Sourced in United States

The Hydraulic Micropositioner is a precision positioning device that utilizes hydraulic technology to enable fine, controlled movements. It is designed to facilitate accurate positioning and adjustment of various components or samples in laboratory and research settings.

Automatically generated - may contain errors

Lab products found in correlation

Picopump, by World Precision Instruments (2 mentions) Recombinant hmgb1, by R&D Systems (1 mentions) Data acquisition system, by Plexon (1 mentions) Sterile charcoal, by Merck Group (1 mentions)

5 protocols using hydraulic micropositioner

Intraspinal HMGB1 Injection in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were anesthetized with a cocktail of ketamine/xylazine (80 and 10mg/kg, respectively). Using aseptic technique, a laminectomy was performed at the T_12–13 vertebral level after which the spinal column was secured via the spinous processes adjacent to the laminectomy site using Adson forceps fixed in a spinal frame. Sterile glass micropipettes (pulled to an external diameter of ~25μm and pre-filled with sterile recombinant HMGB1 (R&D Systems; 500ng/mouse; n=6) or sterile PBS (n=6) were positioned at 0.4mm lateral from midline. From the meningeal surface, pipettes were lowered 0.8mm using a hydraulic micropositioner (David Kopf Instruments, Tujunga, CA). Using a PicoPump (World Precision Instruments, Sarasota, FL), 1μl of solution was injected over a period of 15min. To minimize fluid reflux, pipettes remained in place for 2 additional minutes to allow the injectate to dissipate into the parenchyma. To facilitate localization of the injection sites for anatomical analysis, a small amount of sterile charcoal was placed on the adjacent dura before closing the overlying tissues. Structural testing of recombinant HMGB1 by R&D Systems indicates that the recombinant protein does contain the disulfide bond at Cys23 and Cys45 as well as a free thiol at Cys106. This disulfide form of HMGB1 contains cytokine-stimulating activity (Yang et al., 2012 (link)).

Kigerl K.A., Lai W., Wallace L.M., Yang H, & Popovich P.G. (2017). High mobility group box-1 (HMGB1) is increased in injured mouse spinal cord and can elicit neurotoxic inflammation. Brain, behavior, and immunity, 72, 22-33.

+ Open protocol

+ Expand

Extracellular Recording of Single-Unit Action Potentials

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single unit action potentials were recorded extracellularly with single tungsten electrodes (FHC, Inc., Bowdoinham, ME, USA) after penetration of the dura with a sharp guide tube. The electrode was advanced using a hydraulic micropositioner (David Kopf Instruments, Tujunga, CA, USA). Impedances ranged from 0.5 to 2.8 MΩ. Neuronal activity was amplified and filtered (bandpass 150–5000 Hz). Action potentials in the majority of recorded units were sorted online using the Plexon data acquisition system (Plexon Inc., Dallas, TX, USA). In the first recording sessions action potentials were isolated using a window discriminator (BAK Electronics Inc., Mount Airy, MD, USA). Area MT was identified by its anatomical position, the high proportion of direction-selective cells, and the typical size-eccentricity relationship of RFs. Eye positions were monitored using a video-based eye tracking system (ET-49, Thomas Recording, Giessen, Germany). Eye positions were sampled at 230 Hz, digitized and stored at 200 Hz. Fixation was controlled during the recordings to stay within a window of 1.2° radius around the fixation spot.

Li K., Kozyrev V., Kyllingsbæk S., Treue S., Ditlevsen S, & Bundesen C. (2016). Neurons in Primate Visual Cortex Alternate between Responses to Multiple Stimuli in Their Receptive Field. Frontiers in Computational Neuroscience, 10, 141.

+ Open protocol

+ Expand

Extracellular Single-unit Recording in Primate Visual Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-unit action potentials were recorded extracellularly with single tungsten electrodes (FHC, Bowdoinham, ME) after penetration of the dura with a sharp guide tube. The electrode was advanced using a hydraulic micropositioner (David Kopf Instruments, Tujunga, CA). Electrode impedances ranged from 0.5 to 2.8 MΩ. Neuronal activity was amplified and filtered (bandpass 150–5,000 Hz). Action potentials of the majority of recorded units were sorted online using a Plexon MAP data acquisition system (Plexon, Dallas, TX). In the first recording sessions, action potentials were isolated using a window discriminator (BAK Electronics, Mount Airy, MD). Area MT was identified by its anatomical position, the high proportion of direction-selective cells, and the typical size-eccentricity relationship of RFs. Eye positions were monitored using a video-based eye tracking system (ET-49; Thomas Recording, Giessen, Germany). Eye positions were sampled at 230 Hz, digitized, and stored at 200 Hz. Gaze direction was controlled during the recordings to stay within a window of 1.2° radius around the fixation spot; trials in which the gaze direction left that window were excluded from the analysis.

Kozyrev V., Daliri M.R., Schwedhelm P, & Treue S. (2019). Strategic deployment of feature-based attentional gain in primate visual cortex. PLoS Biology, 17(8), e3000387.

+ Open protocol

+ Expand

Electrophysiological Recording of Neuronal Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the start of each recording session a 23-gauge stainless steel guide tube was positioned in a support grid (1 mm spacing; Crist Instruments) and inserted through the intact dura. A tungsten electrode (8–10 MOhms impedence; FHC) was lowered through the guide tube using a hydraulic micropositioner (KOPF Instruments). Electrophysiological signals were amplified, bandpass filtered, and individual neurons were isolated based on waveform characteristics. Times of spike occurrence were recorded by computer using a 1-µs internal clock. Once a cell was isolated 250–1200 trials were presented in which the location of the eccentric target(s) and the color of the eccentric target varied randomly from trial-to-trial.

Grattan L.E, & Glimcher P.W. (2014). Absence of Spatial Tuning in the Orbitofrontal Cortex. PLoS ONE, 9(11), e112750.

+ Open protocol

+ Expand

Spinal Cord Microinjection in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

All surgical and postoperative care procedures were performed in accordance with The Ohio State University Institutional Animal Care and Use Committee. Adult female Sprague-Dawley rats (~250 grams; n = 56) were randomly assigned to treatment groups (vehicle control, iron, iron+LPS, LPS; n = 10/group), then anesthetized with an intra-peritoneal injection of ketamine (80mg/kg) and xylazine (10mg/kg). Using aseptic technique, a laminectomy was performed at the T8 vertebral level. Custom pulled UV-sterilized glass micropipettes beveled to an outer tip diameter of 25–40μm were loaded with the proper solution and positioned 0.7mm lateral to the dorsal spinal cord midline. Using a hydraulic micropositioner (David Kopf Instruments, Tujunga, CA), pipettes were lowered 1.1mm into the spinal cord. For histological experiments, a 500nl bolus injection was administered to the lateral gray-white matter border using a PicoPump (World Precision Instruments). For tissue RNA experiments, a 200nl bolus injection was administered bilaterally in the lateral gray-white matter border. Injection sites were labeled with sterile charcoal (Sigma), muscles surrounding the laminectomy were sutured, skin was stapled with wound clips, and rats were given 5cc sterile saline (subcutaneous) before being placed into a warmed recovery cage. Two rats died due to complications with anesthesia.

Goldstein E.Z., Church J.S., Pukos N., Gottipati M., Popovich P.G, & McTigue D.M. (2017). Intraspinal TLR4 activation promotes iron storage but does not protect neurons or oligodendrocytes from progressive iron-mediated damage. Experimental neurology, 298(Pt A), 42-56.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!