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Uplsapo 60xw water immersion lens

Manufactured by Olympus
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The UPLSAPO 60XW is a high-numerical aperture water immersion objective lens designed for Olympus microscopes. It provides a large working distance and high resolution for imaging applications that require aqueous sample environments.

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Lab products found in correlation

Ixon 897, by Oxford Instruments (2 mentions) Metamorph 7, by Molecular Devices (2 mentions) Inu zilcs f1, by Tokai Hit (2 mentions) Em01 r488 568 15, by IDEX Corporation (2 mentions) Csu x1, by Yokogawa (2 mentions)

Spelling variants of this product

UPLSAPO (36 citations) 40X water immersion lens (14 citations)

2 protocols using uplsapo 60xw water immersion lens

1

Spinning-Disk Confocal Imaging of PQ-FRET Sensors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells expressing PQ-FRET sensors were imaged using a spinning-disk
confocal microscope: spinning-disk head (CSU-X1, Yokogawa), microscope stand
(IX81, Olympus), 488nm and 561nm laser combiner (ALC, Andor Technology), EMCCD
camera (iXon 897, Andor Technology), laser based focusing system (ZDC1,
Olympus), objective (60×/1.2 NA UPLSAPO 60XW water immersion lens,
Olympus), image acquisition software (MetaMorph 7.7.7.0, Molecular Devices),
stage top humidified chamber (INU-ZILCS-F1, Tokai Hit), whole microscope
37°C chamber (custom designed by HMS Cell Biology machine shop), Semrock
barrier filters (525/40, 607/36), Semrock dicroic mirror (405/488/568/647).
Wavelengths of the excitation laser beam were 488 and 561 nm and fluorescence
for mEGFP and mCherry was detected using 488/568 nm Yokogawa emission filter
(Em01-R488/568-15, Semrock). Images were collected using the maximum field of
view with 512 × 512 image size. Timelapse images were collected with the
frame acquisition rate ranged from 1.0 to 30 s per frame (indicated in the video
legend).
Nakamura F., Song M., Hartwig J.H, & Stossel T.P. (2014). Documentation and Localization of Force-mediated Filamin A Domain Perturbations in Moving Cells. Nature communications, 5, 4656.
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2

Spinning-Disk Confocal Imaging of PQ-FRET Sensors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells expressing PQ-FRET sensors were imaged using a spinning-disk
confocal microscope: spinning-disk head (CSU-X1, Yokogawa), microscope stand
(IX81, Olympus), 488nm and 561nm laser combiner (ALC, Andor Technology), EMCCD
camera (iXon 897, Andor Technology), laser based focusing system (ZDC1,
Olympus), objective (60×/1.2 NA UPLSAPO 60XW water immersion lens,
Olympus), image acquisition software (MetaMorph 7.7.7.0, Molecular Devices),
stage top humidified chamber (INU-ZILCS-F1, Tokai Hit), whole microscope
37°C chamber (custom designed by HMS Cell Biology machine shop), Semrock
barrier filters (525/40, 607/36), Semrock dicroic mirror (405/488/568/647).
Wavelengths of the excitation laser beam were 488 and 561 nm and fluorescence
for mEGFP and mCherry was detected using 488/568 nm Yokogawa emission filter
(Em01-R488/568-15, Semrock). Images were collected using the maximum field of
view with 512 × 512 image size. Timelapse images were collected with the
frame acquisition rate ranged from 1.0 to 30 s per frame (indicated in the video
legend).
Nakamura F., Song M., Hartwig J.H, & Stossel T.P. (2014). Documentation and Localization of Force-mediated Filamin A Domain Perturbations in Moving Cells. Nature communications, 5, 4656.
+ Open protocol
+ Expand

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