Pxpsp

Antibodies used in this study include rabbit antibodies against mGluR1a (Millipore; 1:2000), ERK1/2 (Cell Signaling; 1:2000), pERK1/2 (phosphorylation at T185/Y187 for pERK2; Cell Signaling; 1:1000), GST (Sigma; 1:2000), a phospho-serine/ threonine-proline motif (pS/TP, Abcam, Cambridge, MA; 1:1000), a phospho-MAPK motif PXpSP or pSPXR/K (Cell Signaling; 1:1000), phosphoserine (Invitrogen; 1:1000), phosphothreonine (Invitrogen; 1:1000), Src with phosphorylated tyrosine 416 (pY416, Cell Signaling; 1:1000), Src (Cell Signaling; 1:2000), or actin (Sigma; 1:4000) or mouse antibodies against mGluR1a (BD, Franklin Lakes, NJ; 1:2000), ERK1/2 (Cell Signaling; 1:2000), ERK2 (Abcam; 1:2000), or a phosphothreonine-proline motif (pTP, Cell Signaling; 1:1000). The anti-pY416 antibody reacts with the Src family members when phosphorylated at the conserved activation residue Y416. Pharmacological agents, including (S)-3,5-dihydroxyphenylglycine (DHPG), 3-methyl-aminothiophene dicarboxylic acid (3-MATIDA), and 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene (U0126), were purchased from Tocris Cookson Inc. (Ballwin, MO). All drugs were freshly prepared at the day of experiments.

Antibodies used in this study include rabbit antibodies against mGlu5 (Millipore), ERK1/2 (Cell Signaling Technology, Danvers, MA), pERK1/2 (phosphorylation at T202/Y204 for pERK1; Cell Signaling), GST (Sigma-Aldrich, St. Louis, MO), pS/TP (Abcam), or PXpSP (Cell Signaling), phosphoserine (Invitrogen), or mouse antibodies against mGlu5 (Abcam, Cambridge, MA), ERK1 (Abcam), ERK1/2 (Cell Signaling), or pTP (Cell Signaling). Pharmacological agents, including (RS)-3,5-dihydroxyphenylglycine (DHPG), 3-methyl-aminothiophene dicarboxylic acid (3-MATIDA) and U0126, were purchased from Tocris Cookson Inc. (Ballwin, MO). All drugs were freshly prepared at the day of experiments.