Cd326 apc

Manufactured by BioLegend

CD326-APC is a fluorochrome-conjugated antibody that binds to the CD326 antigen, also known as Epithelial Cell Adhesion Molecule (EpCAM). CD326 is a cell surface glycoprotein expressed on epithelial cells and various types of carcinomas.

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Lab products found in correlation

Cd235a bv421, by BioLegend (1 mentions) Cd71 fitc, by BioLegend (1 mentions) Cd71 pe, by BioLegend (1 mentions) Iscove modified dulbecco media (imdm), by Merck Group (1 mentions) Thermolysin, by Merck Group (1 mentions) Cd34 pe, by BioLegend (1 mentions) Accutase, by Merck Group (1 mentions)

Spelling variants of this product

Rat anti-CD326 (EpCAM)-APC/Cy7 (5 citations) APC anti-mouse CD326/EPCAM) (3 citations) APC antihuman CD326 (EpCAM) antibody (3 citations) APC- conjugated anti-human CD326 (EpCAM) antibody (2 citations) APC anti-mouse CD326 (2 citations) Anti-mEpCAM (CD326)-APC-Cy7 (2 citations)

2 protocols using cd326 apc

Erythroid Lineage Analysis in Human Bone Marrow

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Human bone marrow was stained with a lineage cocktail using FITC-conjugated antibodies to CD2, CD7, CD11b and CD14 and erythroid markers CD71-PE (334105, BioLegend), CD326-APC (324207, BioLegend) and CD235a-BV421 (349131, BioLegend) and DAPI to identify viable cells. Cell sorting was performed on a FACSAria II to collect four populations (CD71⁺CD326⁺CD235a^–, CD71⁺CD326⁺CD235a⁺, CD71⁺CD326^–CD235a⁺ and CD71^–CD326^–CD235a⁺) or sorted on an MA900 where cells were stained with Percp/Cy5.5-conjugated antibodies to CD2, CD7, CD11b and CD14 and erythroid markers CD71-FITC (334103, BioLegend), CD326-APC (BioLegend, clone 9C4) and CD235a-BV421 (BioLegend, clone HI264).

Zhang X., Song B., Carlino M.J., Li G., Ferchen K., Chen M., Thompson E.N., Kain B.N., Schnell D., Thakkar K., Kouril M., Jin K., Hay S.B., Sen S., Bernardicius D., Ma S., Bennett S.N., Croteau J., Salvatori O., Lye M.H., Gillen A.E., Jordan C.T., Singh H., Krause D.S., Salomonis N, & Grimes H.L. (2024). An immunophenotype-coupled transcriptomic atlas of human hematopoietic progenitors. Nature Immunology, 25(4), 703-715.

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Isolating Inner Ear Cell Populations

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Math1-GFP mice and CD-1 mice were euthanized at P1 and P2, respectively, and their temporal bone removed. Inner ear tissues were dissected and placed into 0.5 mg/ml Thermolysin (Sigma-Aldrich Cat# T7902) for 20 minutes at 37°C – 5% CO₂. The Thermolysin was then replaced with Accutase (Sigma-Aldrich Cat# A6964) followed by three rounds of 3 minutes at 37°C and mechanical dissociation. The Accutase was inactivated with IMDM (Sigma-Aldrich, Cat# I6529) supplemented with 5% fetal bovine serum and the cell suspension was filtered through a 35-μm cell strainer to eliminate cell clumps. Dissociated cells were stained with CD326- APC (1:2,000; BioLegend Cat# 118213, RRID:AB_1134105), CD49falexa488 (1:100; BioLegend Cat# 313607, RRID:AB_493634), and CD34-PE (1:200; BioLegend Cat# 128609, RRID:AB_2074602) before FACS.

Hertzano R., Gwilliam K., Rose K., Milon B, & Matern M.S. (2020). Cell Type–Specific Expression Analysis of the Inner Ear: A Technical Report. The Laryngoscope, 131(Suppl 5), S1-S16.

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