Kryptofix2.2.2 k222

Acetonitrile (CH₃CN) and Kryptofix2.2.2 (K₂₂₂) were obtained from Merck (Darmstadt, Germany), and dry dimethyl sulfoxide (DMSO) was purchased from Sigma Aldrich (St. Louis, MO, USA). Sep-Pak light, Accell Plus QMA and Alumina N cartridges were from Waters, Milford, MA, USA. Phenomenex Luna pre-column (C18/2, 50 × 10 mm; 5 µm), Phenomenex Nucleosil columns (C18, 250 × 10 mm; 5 µm and C18, 250 × 4.6 mm) and 0.22 µm Millex GS and LX filters were from Millipore, Billerica, MA, USA. NCA [¹⁸F]fluoride was obtained from a PETtrace 16.5 MeV cyclotron incorporating a high-pressure niobium target (Cyclotek(AUST) Pty. Ltd., Victoria, Australia)) via the ¹⁸O(p,n)¹⁸F nuclear reaction. F-18 separation cartridges (Waters Accell Plus QMA Sep-Pak light, Kent, UK) were pre-conditioned with 0.5 M K₂CO₃ and subsequently rinsed with water. Radio-HPLC analyses were performed using a Shimadzu HPLC (SCL-10AVP system controller, SIL-10ADVP auto injector, LC-10ATVP solvent delivery unit, CV-10AL control valve, DGU-14A degasser, and SPD-10AVPV detector) Q6 coupled to a scintillation detector (Ortec 276 Photomultiplier Base with Preamplifier, Ortec 925-SCINT ACE mate Preamplifier, Amplifier, BIAS supply and SCA, and a Bicron 1M 11/2 Photomultiplier Tube).

Phillygenin (PH) was purchased from Pfeide Biotechnology Co., Ltd. (Chengdu, China). 4-Dimethylaminopyridine and 4-toluene sulfonyl chloride were obtained from Braun Technology Co., Ltd. (Beijing, China). 3-Bromo-1-propanol was obtained from Xien Si Opod Technology Co., Ltd. (Tianjin, China). The herb section of Platycodon grandiflorus (PGS) was obtained from the Zhangzhou Chinese Herbal Medicine Trading Center (Anhui, China). Kryptofix2.2.2 (K2.2.2) was purchased from Merck Corporation (Darmstadt, Germany). H₂¹⁸O was obtained from Dibo Chemical Technology Co., Ltd (Shanghai, China). All other reagents and chemicals were of analytical purity and obtained from Concord Technology (Tianjin, China).

Tracepur H₂O and Kryptofix222 (K₂₂₂) were purchased from Merck. Anhydrous acetonitrile (MeCN), dimethyl sulfoxide (DMSO), oxalic acid and n-octanol were obtained from Sigma-Aldrich; ethanol (EtOH), sodium dihydrogen phosphate (NaH₂PO₄) and disodium hydrogen phosphate (Na₂HPO₄) were obtained from Carl Roth; 0.9% sodium chloride (NaCl)-solution was obtained from VWR (Bruchsal, Germany). Aqueous [¹⁸F]fluoride solution was purchased from EuroPET in Freiburg or the University Hospital Tübingen. The Sep-Pak Accell Plus QMA Carbonate (46 mg) and Sep-Pak C18 Plus SPE Light cartridges (130 mg) were obtained from Waters (Eschborn, Germany). For radioanalytical use, a Dionex UltiMate 3000 system equipped with a Raytest Gabi Star radioactivity detector was used together with a Chromolith Performance (RP-18e, 100–4.6 mm, Merck, Germany) column. All operations were performed with a flow rate of 4 mL/min using H₂O + 0.1% TFA and MeCN + 0.1% TFA as solvents. Radioactivity was measured by an ISOMED 2010 activimeter.