Growth media

Manufactured by Lonza

Sourced in Switzerland, United States

Growth media is a laboratory product designed to provide a nutrient-rich environment for the cultivation and growth of various types of cells, microorganisms, and other biological samples. It serves as a fundamental tool in research, diagnostic, and production applications within the life sciences industry.

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Lab products found in correlation

Hek293t cells, by Merck Group (1 mentions) Nerve growth factor (ngf), by Thermo Fisher Scientific (1 mentions) Horse serum, by Thermo Fisher Scientific (1 mentions) Capsaicin, by R&D Systems (1 mentions) Pdgf bb, by Bachem (1 mentions) Cell culture supplements, by Lonza (1 mentions) Osteoclast precursor cells, by Lonza (1 mentions) Tryple express, by Thermo Fisher Scientific (1 mentions) Hmscs, by Lonza (1 mentions) Lps e coli o111 b4, by Merck Group (1 mentions) Adenosine, by Merck Group (1 mentions) Rolipram, by Merck Group (1 mentions) Inosine, by Merck Group (1 mentions) Cgs21680, by Bio-Techne (1 mentions) Zm241385, by Bio-Techne (1 mentions) Nbmpr, by Bio-Techne (1 mentions) Glutamax icts, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Astrocyte Growth Media (10 citations) EGM-2 Endothelial Cell Growth Media (8 citations) EGM™-2MV growth media (7 citations) Osteoblast Growth Media (4 citations) BEGM bronchial epithelial cell growth media (3 citations) PNGM primary neuron growth media (2 citations) Complete growth media (2 citations) Dental Pulp Stem Cell Growth Media (2 citations) Mammary epithelial cell growth media (2 citations) EGM-2 BulletKit growth media (2 citations)

5 protocols using growth media

Rat Vascular Smooth Muscle Cell Culture

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Primary rat aortic VSMCs, growth media, and cell culture supplements were purchased from Lonza (Basel, Switzerland). Fetal bovine serum for cell culture was supplied by Gibco Life Technologies (Darmstadt, Germany), and PDGF-BB was obtained from Bachem (Weilheim, Germany). Rat phaeochromocytoma cells (PC-12) cells were a kind gift from Prof. Harald Sitte (Medical University of Vienna). Horse serum and NGF were from Fisher Scientific (Vienna, Austria). The A10 cell line was purchased from LGC Standards (Teddington, UK). HEK293T cells were purchased from Merck (Darmstadt, Germany). Sprague Dawley rats (Charles River, MA, USA) were used for harvesting dorsal root ganglia. Biological material was only obtained after sacrificing the animal, which does not require explicit approval by an ethics committee under local jurisdiction. Capsaicin and BCTC were obtained from R&D Systems (Abingdon, UK) and Cayman Chemical (Ann Arbor, MI, USA), respectively. All other chemicals were purchased from Sigma-Aldrich (MO, USA).

Blažević T., Ciotu C.I., Gold-Binder M., Heiss E.H., Fischer M.J, & Dirsch V.M. (2023). Cultured rat aortic vascular smooth muscle cells do not express a functional TRPV1. PLOS ONE, 18(2), e0281191.

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Osteoclast Differentiation with Hemin

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Osteoclast precursor cells and growth media were purchased from Lonza (Walkersville, MD), and differentiated into matured osteoclasts according to the supplier’s instructions. Cells were cultured at 37 °C for 10 days in the absence or presence of 100 μM hemin.

Takeda K., Adhikari R., Yamada K.M, & Dhawan S. (2015). Hemin activation of innate cellular response blocks human immunodeficiency virus type-1-induced osteoclastogenesis. Biochemical and biophysical research communications, 464(1), 7-12.

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hMSC Culture and Labeling with SiC Nanomaterials

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The hMSCs were purchased from Lonza and were used between passage 2 and 10. The growth media was also purchased from Lonza. The seeding density was approximately 5,000 cells/cm² unless specified. Cells were passaged when they reached about 90% confluence with TrypLE Express (Life Technologies) with about 5–8 days between each passage. Labeling with SiC nanoparticles or nanowires was performed without any exogenous transfection agents. The SiC nanomaterials were added to media and allowed to incubate for 4 hours. The adherent cells were washed three times with excessive PBS before detachment or other treatment.

Chen F., Li G., Zhao E.R., Li J., Hableel G., Lemaster J.E., Bai Y., Sen G.L, & Jokerst J.V. (2018). Cellular Toxicity of Silicon Carbide Nanomaterials as a Function of Morphology. Biomaterials, 179, 60-70.

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Investigating Adenosine Signaling Modulators

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CGS 21680, NBMPR, and ZM 241385 were purchased from Tocris Biosciences. Growth media and adenosine deaminase were obtained from Lonza and Worthington Biochemical Corporation respectively. Rolipram, adenosine, inosine, adenosine 5′-[α,β-methylene] diphosphate and LPS (E. coli O111:B4) and all the reagents (unless otherwise stated) were purchased from Sigma-Aldrich.

Welihinda A.A., Kaur M., Raveendran K.S, & Amento E.P. (2017). Enhancement of inosine-mediated A2AR signaling through positive allosteric modulation. Cellular signalling, 42, 227-235.

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Generation of neural progenitor-like cells from human mesenchymal stem cells

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Human MSCs were isolated from bone marrow as described previously with institutional review board (Western IRB) approval and informed consent [Citation2]. MSCs were cultured in growth media (Lonza, MD, USA) supplemented with 5% Plastate™ human platelet lysate (New York Blood Center), 2 U/ml heparin and 2 mM GlutaMAX™I CTS™ (Life Technologies, NY, USA). MSC-NPs were generated by culturing MSCs in neural progenitor maintenance medium (NPMM) (Lonza) supplemented with 20 ng/ml each of epidermal growth factor and basic fibroblast growth factor for 2 weeks. Cells were incubated in a humidified 37°C incubator at 5% CO 2 and 5% O 2 . All MSC-NPs were derived from donors with MS participating in clinical trials (cliniclatrials.gov ID NCT03355365 and NCT03822858), and were quality-tested in compliance with cGMP manufacturing [Citation2] . Conditioned medium (NPCM) was collected from MSC-NPs after 2-3 days in culture, centrifuged for 10 min at 800 × g and 4°C to remove cell debris, and stored at -20°C until use. Twenty-five separate NPCM samples were collected (NPCM1 through NPCM25), and each milliliter represented a cell density range from 30,000 to 170,000 cells/ml.

Harris V.K., Bishop D., Wollowitz J., Carling G., Carlson A.L., Daviaud N, & Sadiq S.A. (2023). Mesenchymal stem cell-derived neural progenitors attenuate proinflammatory microglial activation via paracrine mechanisms. Regenerative medicine, 18(3).

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