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Complete protease inhibitor cocktail complete edta free

Manufactured by Roche
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Complete protease inhibitor cocktail (Complete EDTA-free) is a laboratory reagent designed to inhibit a broad spectrum of proteases. It is an EDTA-free formulation that can be used to preserve protein samples during various experiments and analyses.

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Lab products found in correlation

Galactose, by Thermo Fisher Scientific (4 mentions) Glutamine, by Thermo Fisher Scientific (4 mentions) Phosstop phosphatase inhibitor cocktail, by Roche (4 mentions) Antibody against β actin and hrp labeled secondary antibodies, by Merck Group (3 mentions) Ripa buffer, by Euromedex (3 mentions) Bix02189, by Selleck Chemicals (2 mentions) Xmd8 92, by Selleck Chemicals (2 mentions) 7 aad, by Beckman Coulter (2 mentions) Antibody against β actin, by Merck Group (1 mentions) Erk5 antibody, by Cell Signaling Technology (1 mentions) N n dimethylformamide (dmf), by Merck Group (1 mentions)

Close spelling variants of this product

COmplete mini EDTA-free protease and PhosSTOP phosphatase inhibitor cocktails Complete EDTA-free protease Complete protease cocktail Complete EDTA-free COmplete inhibitor cocktail Complete® inhibitors cocktail Complete protease inhibitor Protease inhibitor cocktail Proteases inhibitor cocktail Cocktail Complete

4 protocols using complete protease inhibitor cocktail complete edta free

1

Immunoblotting Analysis of ERK5 Pathway

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DCA was from Santa Cruz Technologies. Galactose and glutamine were from GIBCO. Human anti-LDLR-PE and IgG were from BD Biosciences and 7AAD from Beckman. The MEK5 inhibitor BIX02189 and the ERK5 inhibitor XMD8–92 were from Selleck. RIPA buffer to prepare protein extracts was from Euromedex. The complete protease inhibitor cocktail (Complete EDTA-free) and the phosphatase inhibitor cocktail (PhosSTOP) were from Roche. ERK5 and MEF2A/C antibodies were from Cell Signaling Technology and Abcam respectively. The antibody against β-Actin and HRP-labeled secondary antibodies were from Sigma.
Khan A.U., Allende-Vega N., Gitenay D., Gerbal-Chaloin S., Gondeau C., Vo D.N., Belkahla S., Orecchioni S., Talarico G., Bertolini F., Bozic M., Valdivielso J.M., Bejjani F., Jariel I., Lopez-Mejia I.C., Fajas L., Lecellier C.H., Hernandez J., Daujat M, & Villalba M. (2017). The PDK1 Inhibitor Dichloroacetate Controls Cholesterol Homeostasis Through the ERK5/MEF2 Pathway. Scientific Reports, 7, 10654.
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2

Protein Extraction and Immunoblotting Protocol

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RIPA buffer to prepare protein extracts was from Euromedex. The complete protease inhibitor cocktail (Complete EDTA-free) and the phosphatase inhibitor cocktail (PhosSTOP) were from Roche. H2O2 was from SIGMA and DCA from Santa Cruz Technologies. Galactose and glutamine were from GIBCO. The antibody against KEAP1 and MEF2 (E-17) were from Santa Cruz Biotechnology. ERK5 antibody was from Cell Signaling Technology. The antibody against β-Actin and HRP-labeled secondary antibodies were from Sigma.
Khan A.U., Rathore M.G., Allende-Vega N., Vo D.N., Belkhala S., Orecchioni S., Talarico G., Bertolini F., Cartron G., Lecellier C.H, & Villalba M. (2015). Human Leukemic Cells performing Oxidative Phosphorylation (OXPHOS) Generate an Antioxidant Response Independently of Reactive Oxygen species (ROS) Production. EBioMedicine, 3, 43-53.
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3

Investigating CD36 and LRP1 Signaling

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DCA was from Santa Cruz Technologies. Galactose and glutamine were from GIBCO. Human anti-CD36-PE, anti-CD36L-PE, anti-LPR1-PE and IgG were from Beckton Dickinson and 7AAD from Beckman (Brea, CA, USA). The MEK5 inhibitor BIX02189 and the ERK5 inhibitor XMD8–92 were from Selleck Chemicals (Radnor, PA, USA). RIPA buffer to prepare protein extracts was from Euromedex (Souffelweyersheim, France). The complete protease inhibitor cocktail (Complete EDTA-free) and the phosphatase inhibitor cocktail (PhosSTOP) were from Roche (Bale, Switzerland). ERK5, ACDVL and MEF2A/C antibodies were from Cell Signaling Technology (Danvers, MA, USA) and Abcam (Cambridge, UK), respectively. The antibody against β-Actin and HRP-labeled secondary antibodies were from Sigma (Burlington, MA, USA).
Khan A.U., Salehi H., Alexia C., Valdivielso J.M., Bozic M., Lopez-Mejia I.C., Fajas L., Gerbal-Chaloin S., Daujat-Chavanieu M., Gitenay D, & Villalba M. (2022). Glucose Starvation or Pyruvate Dehydrogenase Activation Induce a Broad, ERK5-Mediated, Metabolic Remodeling Leading to Fatty Acid Oxidation. Cells, 11(9), 1392.
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4

Analyzing Cellular Signaling Pathways

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DCA was from Santa Cruz Technologies. Galactose and glutamine were from GIBCO. RIPA buffer to prepare protein extracts was from Euromedex. The complete protease inhibitor cocktail (Complete EDTA-free) and the phosphatase inhibitor cocktail (PhosSTOP) were from Roche. H2O2, DMF and MMS were from Sigma. ERK5 and NRF2 antibodies were from Cell Signaling Technology and Santa Cruz respectively. The antibody against β-Actin and HRP-labeled secondary antibodies were from Sigma.
Khan A.U., Allende-Vega N., Gitenay D., Garaude J., Vo D.N., Belkhala S., Gerbal-Chaloin S., Gondeau C., Daujat-Chavanieu M., Delettre C., Orecchioni S., Talarico G., Bertolini F., Anel A., Cuezva J.M., Enriquez J.A., Cartron G., Lecellier C.H., Hernandez J, & Villalba M. (2018). Mitochondrial Complex I activity signals antioxidant response through ERK5. Scientific Reports, 8, 7420.
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