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Gram stain kit

Manufactured by Merck Group
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Sourced in United States, Israel

The Gram stain kit is a laboratory tool used to classify bacteria based on their cell wall structure. The kit contains a series of staining solutions that allow for the differentiation of Gram-positive and Gram-negative bacteria through a standardized staining procedure. The core function of the Gram stain kit is to provide a reliable method for the initial characterization of bacterial samples.

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Lab products found in correlation

Mrs agar, by Merck Group (1 mentions) Sodium alginate, by Merck Group (1 mentions) Calcium chloride, by Merck Group (1 mentions) Tween 80, by Merck Group (1 mentions)

2 protocols using gram stain kit

1

Freeze-dried Lactobacillus gasseri ATCC 19992

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Freeze-dried granules of Lactobacillus gasseri ATCC 19992 ™ were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA). Sodium alginate, Tween® 80, calcium chloride, de Man Rogosa and Sharp Medium (MRS), MRS agar, Gram stain kit, and all the reagents used to perform the in vitro evaluation were purchased from Sigma-Aldrich® (St Louis, MO, USA). The commercial vegetable oil used in this study was bought from a local market in Veracruz (Mexico).
Varela-Pérez A., Romero-Chapol O.O., Castillo-Olmos A.G., García H.S., Suárez-Quiroz M.L., Singh J., Figueroa-Hernández C.Y., Viveros-Contreras R, & Cano-Sarmiento C. (2022). Encapsulation of Lactobacillus gasseri: Characterization, Probiotic Survival, In Vitro Evaluation and Viability in Apple Juice. Foods, 11(5), 740.
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2

Visualizing Bacterial Interaction with Cells

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The cells isolated and cultured as described above were plated on 6-well plates coated with type-I rat tail collagen (Promocell, Sigma, Tel Aviv-Yafo, Israel) and grown for 48–72 h to confluence. To each well, ~106 vegetative bacteria were added and incubated for 3 h. The plates were gently aspirated and washed 6 times with warm PBS, followed by fixation with 10% formaldehyde. The fixed cells were stained using a gram-stain kit (Sigma, Tel Aviv-Yafo, Israel), dried, and photographed under a microscope.
Glinert I., Weiss S., Sittner A., Bar-David E., Ben-Shmuel A., Schlomovitz J., Kobiler D, & Levy H. (2018). Infection with a Nonencapsulated Bacillus anthracis Strain in Rabbits—The Role of Bacterial Adhesion and the Potential for a Safe Live Attenuated Vaccine. Toxins, 10(12), 506.
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