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Fitc anti cd11c antibody

Manufactured by BioLegend

The FITC anti-CD11c antibody is a reagent used in flow cytometry applications. It binds to the CD11c antigen expressed on the surface of dendritic cells, macrophages, and other myeloid cells. The antibody is conjugated with the fluorescent dye FITC, allowing for the detection and analysis of CD11c-positive cells.

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2 protocols using fitc anti cd11c antibody

1

Characterization of BMDC Surface Markers

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The BMDCs were stained by FITC anti-CD11c antibody (Clone: N418), PE anti-CD86 antibody (Clone: GL-1), PE anti-H-2Kd/H-2Dd antibody (Clone: 34–1-2S) and PE anti-I-A/I-E PE antibody (Clone: M5/114.15.2) (BioLegend). The expressions of CD80, CD86, MHC class I and MHC class II on the BMDC were analyzed by flow cytometry (FACSCalibur, BD).
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2

Splenocyte Immunophenotyping by Flow Cytometry

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The splenocytes were treated with fluorescein (FITC) anti-CD11c antibody (clone: N418), PE-cy7 anti-CD11b antibody (clone: M1/70), PE-cy5 anti-CD3e antibody (clone: 145-2C11), FITC anti-CD8a antibody (clone: 5H10-1), antigen-presenting cell (APC) anti-F4/80 antibody (clone: BM8), FITC anti-CD206 antibody (clone: C068C2), and APC anti-CD4 antibody (clone: GK1.5) (BioLegend, Seoul, Korea). The expression of CD3e, CD11b, CD11c, CD8a, and CD4 in splenocytes was analyzed using flow cytometry (FACSCalibur, BD Bioscience, Korea).
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