A venous blood sample was taken approximately 15 min after the experiment at a pathology service located within walking distance from the University by registered nurses trained in venepuncture techniques; all procedures were conducted taking standard infection control precautions. Serum estradiol concentrations were analyzed by Healthscope Pathology Services using an ADVIA Centaur Enhanced Estradiol assay (Siemens), which is a competitive assay that measures serum estradiol concentrations up to 3000 pg/mL (11,010 pmol/L) with a limit of detection of 11.8 pg/mL (43.6 pmol/L). Progesterone levels were analyzed using an ADVIA Centaur Progesterone assay (Siemens), which is a competitive immunoassay that measures serum progesterone concentration up to 60 ng/mL (190.8 nmol/L) with a minimum detectable concentration of 0.21 ng/mL (0.67 nmol/L).
Advia centaur enhanced estradiol assay
Manufactured by Siemens
Sourced in Germany
The ADVIA Centaur Enhanced Estradiol assay is a laboratory equipment product designed to measure the concentration of estradiol in human serum and plasma samples. It is an automated immunoassay that utilizes chemiluminescent technology for the quantitative determination of estradiol levels.
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Lab products found in correlation
3 protocols using advia centaur enhanced estradiol assay
1
Serum Hormone Concentration Analysis
2
Quantifying HMGB1, Insulin, and IL-6 in Follicular Fluid
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HMGB1 concentrations in FF were assayed using a specific research ELISA kit (HMGB1 ELISA, Tecan Trading AG, Switzerland). The intra-assay coefficient of variation (CV) was 5.4%, and the inter-assay CV was 8.2%. The sensitivity of the method was <0.15 ng/mL. Insulin was measured in FF using a specific ultrasensitive ELISA kit (Mercodia Ultrasensitive Insulin ELISA, Mercodia AB, Sweden); the intra-assay CV was 6.5 and the inter-assay CV 7.1%. IL-6 was quantified in FF using an ultrasensitive ELISA method (Human IL6 Quantikine ELISA kit, R&D Systems, Inc. USA) according to the manufacturer's protocol; the intra-assay CV was 3.8% and the inter-assay CV 9.9%. The sensitivity of the method was <0.11 pg/mL. E2 concentrations, obtained from venous blood samples taken the same day as oocyte retrieval, were assayed with the ADVIA Centaur® Enhanced Estradiol Assay (Siemens AG 2010 2018, Germany), an automated, monoclonal, competitive, chemiluminescent immunoassay.
3
Evaluating Menstrual Cycle Hormone Profiles
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A venous blood sample was drawn from each female participant on Day 1 of the experiment at a pathology service located within walking distance from the University.
Serum hormone concentrations were analyzed by Healthscope Pathology Services.
Estradiol levels were analyzed using an ADVIA Centaur Enhanced Estradiol assay (Siemens), which is a competitive assay that measures serum estradiol concentrations up to 3000 pg/mL (11,010 pmol/L) with a limit of detection of 11.8 pg/mL (43.6 pmol/L).
Progesterone levels were analyzed using an ADVIA Centaur Progesterone assay (Siemens), which is a competitive immunoassay that measures serum progesterone concentration up to 60 ng/mL (190.8 nmol/L) with a minimum detectable concentration of 0.21 ng/mL (0.67 nmol/L). Naturally cycling women were invited to participate in the experiment across all phases of the menstrual cycle to achieve wide variance in estradiol and progesterone levels.
The experimenter remained blind to the results of serum analyses for each participant until after the completion of the two-day experimental procedure.
Serum hormone concentrations were analyzed by Healthscope Pathology Services.
Estradiol levels were analyzed using an ADVIA Centaur Enhanced Estradiol assay (Siemens), which is a competitive assay that measures serum estradiol concentrations up to 3000 pg/mL (11,010 pmol/L) with a limit of detection of 11.8 pg/mL (43.6 pmol/L).
Progesterone levels were analyzed using an ADVIA Centaur Progesterone assay (Siemens), which is a competitive immunoassay that measures serum progesterone concentration up to 60 ng/mL (190.8 nmol/L) with a minimum detectable concentration of 0.21 ng/mL (0.67 nmol/L). Naturally cycling women were invited to participate in the experiment across all phases of the menstrual cycle to achieve wide variance in estradiol and progesterone levels.
The experimenter remained blind to the results of serum analyses for each participant until after the completion of the two-day experimental procedure.
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