UV spectra were measured on a Milton Roy Spectronic 300 Array spectrophotometer, and the IR was recorded on a Perkin-Elmer FT-IR 1760x spectrophotometer (Boston, MA, USA). High-resolution electrospray ionization mass spectra (HR-ESI-MS) were recorded with a Bruker micro TOF mass spectrometer (Billerica, MA, USA). NMR spectra were obtained with a Bruker Avance DPX-300 or Avance III HD 500FT-NMR spectrometer (Billerica, MA, USA). MeOH, EtOAc, n-butanol, hexane, CH2Cl2, yeast α-glucosidase, p-NPG, glucose oxidase (GO) assay kit, sodium dodecyl sulfate (SDS), 3-(4,5- dimethyl thiazol-2-yl)-5-diphenyl tetrazolium bromide (MTT), and acarbose were obtained from Sigma-Aldrich (St. Louis, MO, USA). Alpha minimal essentialmedium (α-MEM), fetal bovine serum (FBS), and penicillin-streptomycin (10,000 IU/mL) were purchased from Thermo Fisher Scientific (Grand Island, NY, USA). Insulin (100 IU/mL) was acquired from Biocon (Bangalore, India). Silica gel (SiO2) and TLC plates were obtained from Merck (Darmstadt, Germany), and Sephadex LH-20 was from Pharmacia (Piscataway, NJ, USA).
3 4 5 dimethyl thiazol 2 yl 5 diphenyl tetrazolium bromide mtt
Manufactured by Merck Group
Sourced in United States
3-(4,5-dimethyl thiazol-2-yl)-5-diphenyl tetrazolium bromide (MTT) is a chemical compound used as a colorimetric assay for assessing cell metabolic activity. It is a pale yellow tetrazolium salt that is reduced to a purple formazan product by metabolically active cells.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Etoac, by Merck Group (1 mentions)
Ch2cl2, by Merck Group (1 mentions)
Spectronic 300 array spectrophotometer, by PerkinElmer (1 mentions)
Ft ir 1760x spectrophotometer, by PerkinElmer (1 mentions)
Microtof mass spectrometer, by Bruker (1 mentions)
N butanol, by Merck Group (1 mentions)
Glucose oxidase assay kit, by Merck Group (1 mentions)
Yeast α glucosidase, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Acarbose, by Merck Group (1 mentions)
Silymarin, by Merck Group (1 mentions)
Revertaid reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
Oligo dt primer, by Eurofins (1 mentions)
2 protocols using 3 4 5 dimethyl thiazol 2 yl 5 diphenyl tetrazolium bromide mtt
1
Spectroscopic Characterization of Novel Compounds
2
In Vitro Hepatoprotective Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
Phosphate buffered saline (PBS), 5-Nicotinamide adenine dinucleotide phosphate reduced (NADPH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), fetal bovine serum (FBS), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid) diammonium salt (ABTS) and Dulbecco's modified eagle medium (DMEM), trichloroaceticacid (TCA), thiobarbituricacid (TBA), folin ciocalteau and trypsin were procured from Hi-Media Labs Pvt. Ltd (Mumbai, India). 3-(4, 5-dimethylthiazol-2-yl)-5-diphenyltetrazolium bromide (MTT) and Silymarin were procured from Sigma Aldrich (MO, USA). Kits for serum aspartate aminotransferase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), total protein (TP) and total bilirubin (TB) were procured from Erba diagnostic (Mannheim, Germany). All other chemicals used were analytical grade and obtained from Sigma-Aldrich (MO, USA), Merck (Bangalore, India) and Sd Fine Chem (Mumbai, India). Tri reagent from G Biosciences (St. Louis, USA), oligo dT primer from Eurofins (Bangalore, India), Revert Aid Reverse Transcriptase (Thermo scientific, India), polymerase chain reaction (PCR) master mix from Aristogene (Bangalore, India), Rodent pellet diet from Gold Mohr (Lipton India Ltd, Bangalore, India), Rat (BRL3A) liver cell line was acquired from National Centre for Cell Science (Pune, India).
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Phosphate buffered saline (PBS), 5-Nicotinamide adenine dinucleotide phosphate reduced (NADPH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), fetal bovine serum (FBS), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid) diammonium salt (ABTS) and Dulbecco's modified eagle medium (DMEM), trichloroaceticacid (TCA), thiobarbituricacid (TBA), folin ciocalteau and trypsin were procured from Hi-Media Labs Pvt. Ltd (Mumbai, India). 3-(4, 5-dimethylthiazol-2-yl)-5-diphenyltetrazolium bromide (MTT) and Silymarin were procured from Sigma Aldrich (MO, USA). Kits for serum aspartate aminotransferase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), total protein (TP) and total bilirubin (TB) were procured from Erba diagnostic (Mannheim, Germany). All other chemicals used were analytical grade and obtained from Sigma-Aldrich (MO, USA), Merck (Bangalore, India) and Sd Fine Chem (Mumbai, India). Tri reagent from G Biosciences (St. Louis, USA), oligo dT primer from Eurofins (Bangalore, India), Revert Aid Reverse Transcriptase (Thermo scientific, India), polymerase chain reaction (PCR) master mix from Aristogene (Bangalore, India), Rodent pellet diet from Gold Mohr (Lipton India Ltd, Bangalore, India), Rat (BRL3A) liver cell line was acquired from National Centre for Cell Science (Pune, India).
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