Ba 88a

Following the manufacturer’s instructions, urinary and serum 8-OHdG were analysed in duplicates using highly sensitive and competitive ELISA kits (ab201734; Abcam, Shanghai, China). Serum concentrations were determined by comparison to a standard curve and recorded in ng/l. The intra-and-inter assay coefficients of variation (CV) were 3.5% and 4.5%, respectively. Urinary 8-OHdG concentrations obtained from the standard curves were normalised to creatinine concentrations and recorded as ng/mg Cr.
Serum 8-epi-PGF2α was analysed in duplicate using competitive ELISA kits from Elabscience, Shanghai, China (cat. LogE-EL-0041). The intra-and-inter assay CV were 5.6% and 6.4%, respectively. The absorbance of both 8-epi-PGF2α and 8-OHdG was read at 450 nm on a microplate reader (Bio-Tek ELx808 microplate reader, Hayward, CA, USA).
TAC reagents were obtained from Sigma-Aldrich (Hong Kong, China). Plasma samples were thawed to measure TAC spectrophotometrically at 593 nm using Mindray BA-88A, Wuhan, Hubei, China. The estimation of TAC was based on ferric reducing ability of plasma by following the manufacturer’s instructions. The absorbance was used to obtain the concentrations after comparison to standard curves and recorded in µmol/l.

Serum lipid profile (Triglyceride, Total Cholesterol) and serum level of liver enzymes (SGPT, SGOT) were estimated utilizing specific analytical kit available commercially (Human Diagnostics, Germany). A semi-automatic biochemistry analyzer (Mindray BA-88A, China) was employed to record the data after validating the test procedures in our laboratory setting.

The device used for chemistry analysis (FBS, TC, TAG, and HDL-C) was semi-auto chemistry analyzer BA-88A (S.N WR-04002031; Mindray, Shenzhen, China), and the kits used in this device were for; FBS (Glucose L.S, normal level in the plasma 60–110 mg/dL, and sensitivity: 0.4 mg/dL; Biomed Diagnostics Inc., White City, OR, USA), TC (cholesterol total-L AMS, reference value: less than 200 mg/dL recommended, 200–239 mg/dL upper limit, more than 240 mg/dL high value, sensitivity: 4 mg/dL), TAG (triglyceride glycerol-3-phosphate-oxidase [GPO]-peroxidase [POD], reference value: less than 150 mg/dL desirable, 150–200 upper limit, more than 200 mg/dL high, sensitivity 1 mg/dL), HDL-C (BioMaxima HDL precipitating, reference range: 30–65 mg/dL normal, more than 60 mg/dL desired, less than 35 mg/dL high risk for coronary artery disease, sensitivity: 2.76 mA × dL/mg).