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Endothelial cell growth factor supplement

Manufactured by Merck Group
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Endothelial cell growth factor supplement is a laboratory reagent designed to support the growth and proliferation of endothelial cells in cell culture systems. It provides a source of essential growth factors and nutrients required for the maintenance and expansion of endothelial cell lines.

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Lab products found in correlation

Heparin, by Merck Group (2 mentions) Recombinant human ctla4 ig hum hum, by BioXCell (1 mentions) Recombinant human igg1 fc isotype control, by BioXCell (1 mentions) Newborn calf serum, by Thermo Fisher Scientific (1 mentions) Buprenorphine, by Merck Group (1 mentions) Digitonin, by Thermo Fisher Scientific (1 mentions) Glycerol, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Tris hcl, by Thermo Fisher Scientific (1 mentions) 2 mercaptoethanol bme, by Merck Group (1 mentions) Calcein am, by Thermo Fisher Scientific (1 mentions) Mgcl2, by Merck Group (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Sodium bicarbonate, by Merck Group (1 mentions) Heparin sodium salt, by Merck Group (1 mentions) Cacl2, by Merck Group (1 mentions) Bradford protein assay, by Bio-Rad (1 mentions)

Close spelling variants of this product

Endothelial cell growth factor Endothelial growth supplement Endothelial growth factor Endothelial Cell Supplement

4 protocols using endothelial cell growth factor supplement

1

Endothelial Cell Activation by CTLA4-Ig

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Human umbilical vein endothelial cells were cultured in Medium 199 supplemented with 20% (v/v) fetal bovine serum, 100 U/mL penicillin, 0.1 mg/mL streptomycin, 2 mM l-glutamine, 1 µg/mL heparin (Sigma-Aldrich, St. Louis, MO, USA), and 10 µg/mL endothelial cell growth factor supplement (Sigma-Aldrich, St. Louis, MO, USA). Cells from passage 1 or 4 were used in all experiments. Cells grown to confluence were pretreated with INF-γ (400 U/mL) at 37°C for 24 h. After three washes with PBS, the cells were incubated with medium alone, recombinant human CTLA4-Ig (hum/hum) or recombinant human IgG1-Fc isotype control (both from BioXCell, Lebanon, NH, USA) for an additional 24 h. Unstimulated HUVECs were used as controls. The supernatants were collected for the determination of IDO activity by HPLC.
Forteza M.J., Polyzos K.A., Baumgartner R., Suur B.E., Mussbacher M., Johansson D.K., Hermansson A., Hansson G.K, & Ketelhuth D.F. (2018). Activation of the Regulatory T-Cell/Indoleamine 2,3-Dioxygenase Axis Reduces Vascular Inflammation and Atherosclerosis in Hyperlipidemic Mice. Frontiers in Immunology, 9, 950.
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2

Endothelial Cell Culture Protocol

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2-Mercaptoethanol (BME), CaCl2, MgCl2, buprenorphine, sodium bicarbonate, ascorbic acid, heparin sodium salt, and endothelial cell growth factor supplement were from Sigma-Aldrich (St. Louis, MO, USA). Ficoll-Paque PLUS was from GE Healthcare (Uppsala, Sweden). Penicillin/Streptomycin (P/S), PBS, RPMI, and human serum-type AB (HS) were from Corning (Corning, NY, USA). CCL2 was from PreproTech (Rocky Hill, NJ, USA) and R&D Systems (Minneapolis, MN, USA). Macrophage colony stimulating factor (MCSF-1) was from PreproTech, digitonin from Invitrogen (Carlsbad, CA, USA), EDTA from Promega (Madison, WI, USA), and Bradford protein assay was from Bio-Rad (Hercules, CA, USA). Protein A/G and protein-A agarose beads were from Santa Cruz (Dallas, TX, USA). HEPES was from Technova (Hollister, CA, USA), bovine serum albumin from Life Sciences US biological (Salem, MA, USA), paraformaldehyde (PFA) from Electron Microscopy Sciences (Hatfield, PA, USA) and nonfat dry milk was from LabScientific (Highlands, NJ, USA). Fetal bovine serum (FBS), M199, HBSS, TRIS-HCL, NaCl, calcein-AM, glycerol, Medium 199 (M199), L-glutamine, and new born calf serum were from Thermo Fischer Scientific (Waltham, MA, USA). Bovine brain extract was from Clonetics/Lonza (Walkersville, MD, USA).
Jaureguiberry-Bravo M., Lopez L, & Berman J.W. (2018). Buprenorphine decreases CCL2-mediated migration of CD14+CD16+ monocytes. Journal of leukocyte biology, 104(6), 1049-1059.
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3

Culturing Human Brain Microvascular Endothelial Cells

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The Human Brain Microvascular Endothelial Cells (HBMVEC) were obtained from iXCells Biotechnologies (San Diego, CA 92121, USA). The cells were cultured according to the standard protocol given by the manufacturer. The cells were grown in 96-well plates in CS-C medium (Sigma, St Louis, MO, USA) supplemented with 5% FBS (Sigma), 1% endothelial cell growth factor supplement (Sigma), and 1% penicillin solution (Sigma), and incubated at 37 °C in a humidified 5% CO2 incubator.
Wójciak M., Feldo M., Borowski G., Kubrak T., Płachno B.J, & Sowa I. (2022). Antioxidant Potential of Diosmin and Diosmetin against Oxidative Stress in Endothelial Cells. Molecules, 27(23), 8232.
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4

HUVEC Cell Culture Protocol

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HUVEC were purchased from the American Type Culture Collection (Manassas, VA, USA) and serially passaged in M199 (Euroclone, Milan, Italy) containing 10% fetal bovine serum (Euroclone, Milan, Italy), 2 mM glutamine (Euroclone, Milan, Italy), 1 mM sodium pyruvate (Sigma-Aldrich, St. Louis, MO, USA), 5 U/mL heparin (Sigma-Aldrich, St. Louis, MO, USA) and 150 µg/mL endothelial cell growth factor supplement (Sigma-Aldrich, St. Louis, MO, USA) on collagen-coated dishes (50 µg/mL) (Sigma-Aldrich, St. Louis, MO, USA).
Castiglioni S., Locatelli L., Fedele G., Cazzaniga A., Malucelli E., Iotti S, & Maier J.A. (2023). The Interplay between TRPM7 and MagT1 in Maintaining Endothelial Magnesium Homeostasis. Membranes, 13(3), 286.
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