An undifferentiated human iPSC line (Thermo Fisher Scientific) was routinely maintained in complete Essential 8 (E8) cell culture medium (Thermo Fisher Scientific) on 6-well plates coated with 1 : 100 growth factor–reduced Matrigel (R&D Systems). The cells were passaged every 3–4 days using 0.5 mM EDTA in D-PBS (Thermo Fisher Scientific) and re-plated in E8 medium supplemented with 2 μM of the ROCK inhibitor Thiazovivin (Stratech Scientific) for the first 24 h following passaging. Human cardiac microvascular endothelial cells (hCMEC; PromoCell) were grown in PromoCell Cell Growth Medium and passaged using PromoCell DetachKit. The media was changed every two to three days. Human cardiac fibroblasts (hCFib; kindly provided by Prof. Terracciano) were cultured in DMEM high glucose (Thermo Fisher Scientific) supplement with 10% (v/v) FBS (Thermo Fisher Scientific) and dissociated using 0.25% trypsin (Sigma-Aldrich).
Human ipsc line
Manufactured by Thermo Fisher Scientific
The Human iPSC line is a type of induced pluripotent stem cell (iPSC) derived from human cells. iPSCs are reprogrammed from adult somatic cells, such as skin or blood cells, and can differentiate into a variety of cell types. This product provides a source of human iPSCs for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Hcmec, by PromoCell (2 mentions)
Complete essential 8 cell culture medium, by Thermo Fisher Scientific (2 mentions)
Growth factor reduced matrigel, by R&D Systems (2 mentions)
High glucose dmem, by Thermo Fisher Scientific (2 mentions)
Trypsin, by Merck Group (2 mentions)
Detachkit, by PromoCell (2 mentions)
2 protocols using human ipsc line
1
Culturing iPSCs, hCMECs, and hCFibs
2
Culturing iPSCs, hCMECs, and hCFibs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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An undifferentiated human iPSC line (Thermo Fisher Scientific) was routinely maintained in complete Essential 8 (E8) cell culture medium (Thermo Fisher Scientific) on 6-well plates coated with 1 : 100 growth factor–reduced Matrigel (R&D Systems). The cells were passaged every 3–4 days using 0.5 mM EDTA in D-PBS (Thermo Fisher Scientific) and re-plated in E8 medium supplemented with 2 μM of the ROCK inhibitor Thiazovivin (Stratech Scientific) for the first 24 h following passaging. Human cardiac microvascular endothelial cells (hCMEC; PromoCell) were grown in PromoCell Cell Growth Medium and passaged using PromoCell DetachKit. The media was changed every two to three days. Human cardiac fibroblasts (hCFib; kindly provided by Prof. Terracciano) were cultured in DMEM high glucose (Thermo Fisher Scientific) supplement with 10% (v/v) FBS (Thermo Fisher Scientific) and dissociated using 0.25% trypsin (Sigma-Aldrich).
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