SA solution, or SA solution enriched by BP either with or without intact cells, or BP suspension alone, was deposited on MALDI steel target plate and analysed by TESCAN VEGA TS 5136 XM. Image analysis and measurement of crystal parameters were performed in ImageJ software (U. S. National Institutes of Health, Bethesda, Maryland, USA).
Vega ts 5136xm
Manufactured by TESCAN
Sourced in Czechia
The VEGA TS 5136XM is a scanning electron microscope (SEM) produced by TESCAN. It is designed for high-resolution imaging and analysis of a wide range of samples. The core function of the VEGA TS 5136XM is to provide detailed, high-quality images and data about the surface structure and composition of materials at the micro- and nano-scale.
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Lab products found in correlation
4 protocols using vega ts 5136xm
1
MALDI-TOF Analysis of SA Samples
2
Mycelia Morphological Analysis by SEM
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Scanning electron microscopy (SEM) was performed to determine morphological changes in the mycelia on the treated media. First, the growing mycelia (3mm × 5mm) on medium treated with SE-III were sampled with a double-sided knife, and samples for SEM were fixed in 2.5% glutaraldehyde stationary liquid for 12 h. Next, samples were irrigation three times with 0.1 mol L-1 phosphate buffer. The samples were dehydrated with a gradient concentration of alcohol systematically at 30%, 50%, 70%, 80%, 90%, 95%, and 100% with 15 min at each gradient. Anhydrous acetone was then applied three times for dehydration and each bout lasted 15 min. A critical point drying apparatus (EMS 850, Pennsylvania, USA) was used to dry the sample completely. The dried sample was then pasted onto the sample stage to be sputtered with gold (Hitachi ion sputter E-1010, Tokyo, Japan) and observed via SEM (Tescan VEGA TS 5136XM, Brno, Czech) under 2,500× magnification.
3
SEM Imaging of Fiber Morphology
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SEM imaging was conducted with a Tescan VEGA TS 5136XM, TESCAN Company, Brno, Czech Republic, to investigate the samples’ morphology. All samples were sputter-coated with a nominally 20 nm thin gold film using a Quorum Tech Q150R S, Quorum Company, East Sussex, UK, sputter coater. The fiber diameters and their distribution were measured using the ImageJ 1.52a software.
4
Comparative SEM Analysis of Lingual Papillae
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Systematic sampling strategies were applied for the collection of the research material. Tissue samples for SEM [30 (link)] were taken from apex, body and root parts of the 5 tongues (8 samples from one half of each tongue). Each sample contained lingual papillae to compare with the studied species of Felidae family.
Samples of lingual papillae were placed in 2.0% glutaraldehyde dissolved in 0.1M phosphate buffer at pH 7.4. Further sample analyses were performed based on methodology of Čížek et al. [31 (link)]. SEM samples were dried at the critical point (Bal-tec CPD 030 Critical Point Dryer, Leica Biosystems, Wetzlar, Germany) and then gold-coated (Balzers SCD 040 by current 30 mA for 4 min, Balzers, Lichtenstein). Photographic documentation was obtained using Tescan VEGA TS 5,136 XM (Tescan, s.r.o., Brno-Kohoutovice, Czech Republic) scanning electron microscope in a high vacuum and accelerated voltage 20 kV using an SE detector.
In this publication, all anatomical and histological terms are used based on the terminology of Nomina Anatomica Veterinaria (2017) [32 ] and Nomina Histologica Veterinaria (2017) [33 ].
Samples of lingual papillae were placed in 2.0% glutaraldehyde dissolved in 0.1M phosphate buffer at pH 7.4. Further sample analyses were performed based on methodology of Čížek et al. [31 (link)]. SEM samples were dried at the critical point (Bal-tec CPD 030 Critical Point Dryer, Leica Biosystems, Wetzlar, Germany) and then gold-coated (Balzers SCD 040 by current 30 mA for 4 min, Balzers, Lichtenstein). Photographic documentation was obtained using Tescan VEGA TS 5,136 XM (Tescan, s.r.o., Brno-Kohoutovice, Czech Republic) scanning electron microscope in a high vacuum and accelerated voltage 20 kV using an SE detector.
In this publication, all anatomical and histological terms are used based on the terminology of Nomina Anatomica Veterinaria (2017) [32 ] and Nomina Histologica Veterinaria (2017) [33 ].
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