Primary mouse embryonic fibroblasts

Manufactured by Merck Group

Primary mouse embryonic fibroblasts are a type of cell culture derived from the connective tissue of mouse embryos. They are a widely used tool in various research applications.

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Lab products found in correlation

Retinoic acid, by Merck Group (2 mentions) Facsaria 3, by BD (1 mentions) Smoothened agonist sag, by Merck Group (1 mentions)

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Mouse embryonic fibroblasts (6 citations) Mouse fibroblasts (3 citations) Mitomycin C-treated primary mouse embryonic fibroblasts (2 citations)

2 protocols using primary mouse embryonic fibroblasts

Differentiating Embryonic Stem Cells into Motor Neurons

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Mouse embryonic stem cells expressing GFP under the motor neuron (MN)-specific promoter HB9 (HBG3 cells; gift from Tom Jessell) were cultured on primary mouse embryonic fibroblasts (Millipore). For differentiation into MNs, cells were treated with trypsin and resuspended in DFK10 culture medium consisting of knockout DMEM/F12, 10% knockout serum replacement, 1% N2, 0.5% L-glutamine, 0.5% glucose (30% in water), and 0.0016% 2-mercaptoethanol. The cells were plated on non-adherent Petri dishes to allow formation of embryoid bodies. After 1 d of recovery, 2μM retinoic acid (Sigma) and 1μM Smoothened Agonist (SAG) (Millipore) were added to the medium every day for 5 days. Embryoid bodies were then dissociated with papain and sorted using the FACSAria™ III (BD Biosciences).

Glajch K.E., Ferraiuolo L., Mueller K.A., Stopford M.J., Prabhkar V., Gravanis A., Shaw P.J, & Sadri-Vakili G. (2016). MicroNeurotrophins Improve Survival in Motor Neuron-Astrocyte Co-Cultures but Do Not Improve Disease Phenotypes in a Mutant SOD1 Mouse Model of Amyotrophic Lateral Sclerosis. PLoS ONE, 11(10), e0164103.

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Differentiation of Mouse Embryonic Stem Cells into Motor Neurons

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Mouse embryonic stem cells expressing GFP under the MN-specific promoter
HB9 (HBG3 cells; kind gift from Tom Jessell, Columbia University, New York) were
cultured on primary mouse embryonic fibroblasts (Millipore). For differentiation
into MNs, cells were lifted with trypsin and re-suspended in DFK10 culture
medium consisting of knockout DMEM/F12, 10% knockout serum replacement,
1% N2, 0.5% L-glutamine, 0.5% glucose (30% in
water), and 0.0016% 2-mercaptoethanol. The cells were plated on
non-adherent Petri dishes to allow formation of embryoid bodies. After 1 day of
recovery, 2 µM retinoic acid (Sigma) and 2 µM purmorphamine
(Calbiochem) were added freshly every day with new medium. After 5 days of
differentiation, the embryoid bodies were dissociated and sorted for GFP on a BD
FACSVantage/DiVa sorter.

Heilman P.L., Song S., Miranda C.J., Meyer K., Srivastava A.K., Knapp A., Wier C.G., Kaspar B.K, & Kolb S.J. (2017). HSPB1 mutations causing hereditary neuropathy in humans disrupt non-cell autonomous protection of motor neurons. Experimental neurology, 297, 101-109.

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