Melphalan

Manufactured by Santa Cruz Biotechnology

Sourced in Macao, United States

Melphalan is a chemotherapeutic agent used in the treatment of various types of cancer. It functions as an alkylating agent, interfering with DNA synthesis and cell division. Melphalan is commonly used in the management of multiple myeloma, ovarian cancer, and other hematological malignancies.

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Lab products found in correlation

Cisplatin, by Bio-Techne (1 mentions) Topotecan, by Merck Group (1 mentions) Etoposide, by Santa Cruz Biotechnology (1 mentions) 4 hydroperoxy cyclophosphamide, by Santa Cruz Biotechnology (1 mentions) Vincristine sulfate, by Bio-Techne (1 mentions) Sn 38, by Bio-Techne (1 mentions) Lenalidomide, by Santa Cruz Biotechnology (1 mentions) Z vad fmk, by Medical & Biological Laboratories (1 mentions) Facsverse, by BD (1 mentions)

2 protocols using melphalan

Synthesis and Procurement of Cancer Drugs

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BO-1055 was synthesized at Dr. Tsann Long Su's laboratory at the Institute of Biomedical Sciences (IBMS), Academia Sinica, Taipei, Taiwan. PU-H71 was synthesized at Dr. Gabriela Chiosis' laboratory at Memorial Sloan-Kettering Cancer Center (MSKCC), New York, NY, USA. Topotecan was purchased from Sigma-Aldrich (Saint Louis, MO), etoposide, melphalan and 4-hydroperoxy cyclophosphamide (4-HC) were purchased from Santa Cruz Biotechnology (Dallas, TX). SN38, Bendamustine hydrochloride, vincristine sulfate and cisplatin were purchased from Tocris (Bio-Techne, Minneapolis, MN). Doxorubicin and cyclophosphamide were purchased from the pharmacy at MSKCC.

Ambati S.R., Shieh J.H., Pera B., Lopes E.C., Chaudhry A., Wong E.W., Saxena A., Su T.L, & Moore M.A. (2016). BO-1055, a novel DNA cross-linking agent with remarkable low myelotoxicity shows potent activity in sarcoma models. Oncotarget, 7(28), 43062-43075.

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Cytotoxicity and Apoptosis Assay Protocol

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Cytotoxicity of the compounds was analyzed by Cell Counting Kit-8 (Dohjin Chemicals, Kumamoto, Japan) or trypan blue dye exclusion assay according to the manufacturer's protocol. The MEBCYTO Apoptosis kit was used to detect apoptosis by flow cytometry using FACSVerse (BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer's protocol. Melphalan and lenalidomide were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA) and Sigma-Aldrich; Merck KGaA, (Darmstadt, Germany), respectively. A pan-caspase inhibitor, ZVAD-fmk (Medical and Biological Laboratories, Nagoya, Japan), was utilized in some experiment at a concentration of 32 nM.

Fujii E., Inada Y., Kakoki M., Nishimura N., Endo S., Fujiwara S., Wada N., Kawano Y., Okuno Y., Sugimoto T, & Hata H. (2018). Bufalin induces DNA damage response under hypoxic condition in myeloma cells. Oncology Letters, 15(5), 6443-6449.

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