The following antibodies were used: mouse monoclonal anti-β-actin (cat. no. A1978, 1:10,000; Sigma-Aldrich), rabbit polyclonal anti-FLAG (cat. no. 2368, 1:1000; Cell Signaling), mouse monoclonal anti-PARP-1 (sc8007, 1:1000; Santa Cruz), rabbit polyclonal anti-calnexin (sc11397, 1:1000; Santa Cruz), mouse monoclonal anti-V5 antibody (R960-25, 1:4000; Invitrogen), rat monoclonal anti-HA antibody (12158167001, 1:4000; Roche), rabbit polyclonal anti-HA antibody (71-5500, 1:1000 Invitrogen), goat polyclonal anti-calnexin (sc-6465, 1:200; Santa Cruz), sheep anti-TGN46 (AHP500, 1:200 AbD Serotec) and mouse monoclonal anti-GM130 antibody (610823, 1:200; BD Biosciences). Goat anti-mouse (cat. no. 31430, 1:5,000) and goat anti-rabbit IgG conjugated to horseradish peroxidase (31460, 1:5,000) were from Thermo Fischer Scientific. The following secondary antibodies were used for indirect immuno-fluorescence: FITC-conjugated donkey anti-sheep/goat (STAR88F, 1:200; AbD Serotec), Cy3-conjugated donkey anti-mouse IgG (715-165-150, 1:200; Jackson ImmunoResearch), Cy5-conjugated donkey anti-rabbit IgG (711-175-152, 1:200; Jackson ImmunoResearch), Cy2-conjugated donkey anti-mouse IgG (715-225-150, 1:200; Jackson ImmunoResearch), Cy5-conjugated donkey anti-goat IgG (705-175-147, 1:200; Jackson ImmunoResearch), Cy3-conjugated donkey anti-rabbit IgG (711-165-152, 1:200; Jackson ImmunoResearch).
Goat polyclonal anti calnexin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Goat polyclonal anti-calnexin is a laboratory reagent used for the detection and analysis of calnexin, a calcium-binding protein found in the endoplasmic reticulum. It is a polyclonal antibody raised in goats and can be used in various immunochemical techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to identify and quantify calnexin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal anti v5 antibody, by Thermo Fisher Scientific (1 mentions)
Sheep anti tgn46, by Bio-Rad (1 mentions)
Rabbit polyclonal anti flag, by Merck Group (1 mentions)
Mouse monoclonal anti parp 1, by Santa Cruz Biotechnology (1 mentions)
Anti ha rat monoclonal antibody, by Roche (1 mentions)
Mouse monoclonal anti gm130 antibody, by BD (1 mentions)
Goat anti rabbit igg conjugated to horseradish peroxidase, by Thermo Fisher Scientific (1 mentions)
Cy5 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Cy5 conjugated donkey anti goat igg, by Jackson ImmunoResearch (1 mentions)
Rabbit polyclonal anti calnexin, by Santa Cruz Biotechnology (1 mentions)
Cy2 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Cy3 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Cy3 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Polyclonal rabbit anti actin, by Merck Group (1 mentions)
2 protocols using goat polyclonal anti calnexin
1
Antibody Characterization and Validation
2
Antibody Detection and Quantification
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The following primary antibodies were used: goat polyclonal anti-AQP4 (C-19) (sc-9888, 1:500) rabbit polyclonal anti-AQP4 AQP4 (H-80) (sc-20812), goat polyclonal anti-calnexin, (sc6465, 1:250) (Santa Cruz Biotechnology, Dallas, TX, USA); rabbit polyclonal anti-actin (A42066, 1:500) (www.sigmaaldrich.com ); goat anti-human IgG antibody, biotin conjugate (AP112B, 1:7000) (www.merckmillipore.com ).
The secondary antibodies used for immunofluorescence analysis were: donkey anti-goat Alexa Fluor 488-conjugated (A11055) and goat anti-human Alexa Fluor 488-conjugated (A11013) (www.thermofisher.com ).
The following secondary antibodies were used for Western blot: donkey anti-goat IgG-HRP (sc-2020) and goat anti-rabbit IgG-HRP (sc-2004).
The secondary antibody used for ELISA was streptavidin, peroxidase conjugated (SA202, 1:1000) (www.merckmillipore.com ).
The secondary antibodies used for immunofluorescence analysis were: donkey anti-goat Alexa Fluor 488-conjugated (A11055) and goat anti-human Alexa Fluor 488-conjugated (A11013) (
The following secondary antibodies were used for Western blot: donkey anti-goat IgG-HRP (sc-2020) and goat anti-rabbit IgG-HRP (sc-2004).
The secondary antibody used for ELISA was streptavidin, peroxidase conjugated (SA202, 1:1000) (
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