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Mouse anti cd163

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The Mouse anti-CD163 is a laboratory reagent used for the identification and detection of the CD163 protein expressed on the surface of certain cells. CD163 is a scavenger receptor that plays a role in the clearance of hemoglobin-haptoglobin complexes. This product can be used in various immunological and cell biology applications.

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Lab products found in correlation

E1000, by Nikon (2 mentions) Rabbit anti ifnγ, by Abcam (2 mentions) Rabbit anti tnf, by Bioss Antibodies (2 mentions) Rabbit anti il 4, by Abcam (2 mentions) Rabbit anti il 10, by Abcam (2 mentions) Rabbit anti cd3, by Agilent Technologies (2 mentions) Mouse anti calprotectin, by Thermo Fisher Scientific (2 mentions) Ham56, by Enzo Life Sciences (2 mentions) Flowview 1000, by Nikon (2 mentions) Anti rabbit and anti mouse secondary antibodies, by Jackson ImmunoResearch (2 mentions) Prolong gold mounting medium, by Thermo Fisher Scientific (2 mentions) Confocal microscope, by Olympus (2 mentions) Mouse anti cd68, by Thermo Fisher Scientific (1 mentions) Rat anti pnad, by BioLegend (1 mentions) Goat anti lyve 1, by R&D Systems (1 mentions) Rabbit anti collagen 1, by Abcam (1 mentions) Retriever, by Electron Microscopy Sciences (1 mentions) Rabbit polyclonal anti cd3, by Agilent Technologies (1 mentions)

Close spelling variants of this product

CD163 (33 citations) Anti-CD163 (3 citations) Anti-mouse CD163- PerCP (3 citations) Anti-CD163 (clone 10D6; mouse (2 citations)

3 protocols using mouse anti cd163

1

Immunohistochemical Profiling of Immune Cells

Cited 1 time
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Immunohistochemistry was performed on 5 μm-thick FFPE tissue sections as previously indicated1 (link),39 (link). Tissues were stained with combinations of primary antibodies including polyclonal rabbit anti-IFNγ (Abcam, Cambridge, MA), rabbit anti-TNF (Bioss Antibodies, Boston, MA), rabbit anti-IL-4 (Abcam), rabbit anti-IL-10 (Abcam), rabbit anti-CD3 (Agilent, Santa Clara, CA), mouse anti-calprotectin (clone: MAC378, ThermoFisher), mouse anti-CD163 (clone: 1D6, ThermoFisher), and mouse anti-human alveolar macrophage antibody (HAM56, Enzo Life Sciences, Farmingdale, NY). Tissues were subsequently stained with appropriate anti-rabbit and anti-mouse secondary antibodies (Jackson ImmunoResearch, West Grove, PA) for 1 hr at room temp, and coverslips were mounted with DAPI-containing Prolong Gold mounting medium (ThermoFisher). Cells were imaged on an Olympus confocal microscope (Olympus, Waltham, MA) running FlowView 1000 software maintained by the University of Pittsburgh’s Department of Microbiology and Molecular Genetics, or a Nikon e1000 epifluorescence microscope running Nikon Elements (Nikon Instruments, Melville, NY). Images were annotated with Photoshop CS5.1 (Adobe Systems, San Jose, CA) and projections of z-stacks were made with the FIJI build of ImageJ42 (link). Quantitative image analysis was performed with CellProfiler43 (link).
Gideon H.P., Phuah J., Junecko B.A, & Mattila J.T. (2019). Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques. Mucosal immunology, 12(6), 1370-1381.
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2

Comprehensive Immunohistochemical Analysis of Lymph Nodes

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed as previously indicated [73 (link), 82 (link)] on formalin-fixed paraffin-embedded (FFPE) LNs obtained at necropsy. Briefly, sections were deparaffinized and antigen retrieval was performed using a Retriever (Electron Microscopy Services, Hatfield, PA) in Tris-EDTA-Tween-80 buffer 73. Sections were stained for Tcells/B cells/dendritic cells (polyclonal rabbit anti-CD3, Dako, Santa Clara, CA; polyclonal rabbit anti-CD20, Thermo Fisher Scientific, Pittsburgh, PA; mouse-anti-CD11c, Leica Microsystems, Buffalo Grove, IL), macrophage subsets (mouse anti-CD68, Thermo Fisher; rabbit anti-DC-SIGN, ProSci Inc, Poway, CA; mouse anti-CD163, Thermo Fisher), LN vascular and structural aspects (Goat anti-LYVE-1, R&D Systems, Minneapolis, MN; rat-anti PNAd, BioLegend, San Diego, CA), and LN conduit systems (visualized by staining for rabbit anti-collagen 1 [Abcam, Cambridge, MA]). Primary antibodies were visualized with species- and isotype-specific secondary antibodies purchased from Jackson ImmunoResearch (West Grove, PA). Auramine rhodamine was performed as previously indicated [73 (link)] using reagents from BD Biosciences (San Jose, CA). Images were acquired at 20x magnification with a Nikon e1000 widefield microscope (Nikon, Melville, NY) with Nikon Elements.
Ganchua S.K., Cadena A.M., Maiello P., Gideon H.P., Myers A.J., Junecko B.F., Klein E.C., Lin P.L., Mattila J.T, & Flynn J.L. (2018). Lymph nodes are sites of prolonged bacterial persistence during Mycobacterium tuberculosis infection in macaques. PLoS Pathogens, 14(11), e1007337.
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3

Immunohistochemical Profiling of Immune Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed on 5 μm-thick FFPE tissue sections as previously indicated1 (link),39 (link). Tissues were stained with combinations of primary antibodies including polyclonal rabbit anti-IFNγ (Abcam, Cambridge, MA), rabbit anti-TNF (Bioss Antibodies, Boston, MA), rabbit anti-IL-4 (Abcam), rabbit anti-IL-10 (Abcam), rabbit anti-CD3 (Agilent, Santa Clara, CA), mouse anti-calprotectin (clone: MAC378, ThermoFisher), mouse anti-CD163 (clone: 1D6, ThermoFisher), and mouse anti-human alveolar macrophage antibody (HAM56, Enzo Life Sciences, Farmingdale, NY). Tissues were subsequently stained with appropriate anti-rabbit and anti-mouse secondary antibodies (Jackson ImmunoResearch, West Grove, PA) for 1 hr at room temp, and coverslips were mounted with DAPI-containing Prolong Gold mounting medium (ThermoFisher). Cells were imaged on an Olympus confocal microscope (Olympus, Waltham, MA) running FlowView 1000 software maintained by the University of Pittsburgh’s Department of Microbiology and Molecular Genetics, or a Nikon e1000 epifluorescence microscope running Nikon Elements (Nikon Instruments, Melville, NY). Images were annotated with Photoshop CS5.1 (Adobe Systems, San Jose, CA) and projections of z-stacks were made with the FIJI build of ImageJ42 (link). Quantitative image analysis was performed with CellProfiler43 (link).
Gideon H.P., Phuah J., Junecko B.A, & Mattila J.T. (2019). Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques. Mucosal immunology, 12(6), 1370-1381.
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