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Xpenicillin streptomycin

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XPenicillin-Streptomycin is a sterile solution containing penicillin and streptomycin, two broad-spectrum antibiotics commonly used in cell culture applications. The product provides a convenient and consistent way to supplement cell culture media with these antimicrobial agents.

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Lab products found in correlation

Hes qualified matrigel, by Corning (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Trypsin, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) Relesr, by STEMCELL (2 mentions) Mtesr1, by STEMCELL (2 mentions) Hek293t 17 cells, by American Type Culture Collection (2 mentions)

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Streptomycin (865 citations)

2 protocols using xpenicillin streptomycin

1

Targeted CRISPR Repression in hESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
H1 hESCs (WiCell, WA-01) were maintained in mTeSR1 (StemCell
Technologies, Inc., 05850) on hES-qualified Matrigel (Corning, 354277)
coated plates. Cells were fed daily and split with ReLeSR (StemCell
Technologies, Inc., 05872) every 4–5 days in mTeSR1. H1 hESCs were
used to generate the targeted H1-AAVS1-TetOn-dCas9-KRAB hES cell line used
in this study. H1-AAVS1-TetOn-dCas9-KRAB hESCs were maintained similarly to
H1 hESCs.
HEK293T/17 cells (ATCC, CRL-11268) were maintained in DMEM (Thermo,
11965) supplemented with 10% FBS (Thermo, 10437), 1X GlutaMAX (Thermo,
35050), 1X non-essential amino acids (NEAA; Thermo, 11140), and 1X
Penicillin-Streptomycin (Corning, 30–002-CI). Cells were split with
0.25% Trypsin (Thermo, 25200) every 3–5 days.
Genga R.M., Kernfeld E.M., Parsi K.M., Parsons T.J., Ziller M.J, & Maehr R. (2019). Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Cell reports, 27(3), 708-718.e10.
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2

Targeted CRISPR Repression in hESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
H1 hESCs (WiCell, WA-01) were maintained in mTeSR1 (StemCell
Technologies, Inc., 05850) on hES-qualified Matrigel (Corning, 354277)
coated plates. Cells were fed daily and split with ReLeSR (StemCell
Technologies, Inc., 05872) every 4–5 days in mTeSR1. H1 hESCs were
used to generate the targeted H1-AAVS1-TetOn-dCas9-KRAB hES cell line used
in this study. H1-AAVS1-TetOn-dCas9-KRAB hESCs were maintained similarly to
H1 hESCs.
HEK293T/17 cells (ATCC, CRL-11268) were maintained in DMEM (Thermo,
11965) supplemented with 10% FBS (Thermo, 10437), 1X GlutaMAX (Thermo,
35050), 1X non-essential amino acids (NEAA; Thermo, 11140), and 1X
Penicillin-Streptomycin (Corning, 30–002-CI). Cells were split with
0.25% Trypsin (Thermo, 25200) every 3–5 days.
Genga R.M., Kernfeld E.M., Parsi K.M., Parsons T.J., Ziller M.J, & Maehr R. (2019). Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Cell reports, 27(3), 708-718.e10.
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+ Expand

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