Immobilin fl pvdf membranes

Manufactured by Merck Group

Immobilin-FL PVDF membranes are a type of polyvinylidene fluoride (PVDF) membrane used in various laboratory applications. PVDF is a versatile material known for its chemical and thermal resistance. The Immobilin-FL membranes are designed for use in applications such as Western blotting, dot blotting, and protein and nucleic acid transfer and immobilization.

Automatically generated - may contain errors

Lab products found in correlation

Anti flag, by Merck Group (2 mentions) Fish skin gelatin, by Merck Group (2 mentions) Mini protean gel system, by Bio-Rad (2 mentions) Trans blot sd semi dry transfer cell, by Bio-Rad (2 mentions) Anti ogt, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

PVDF membranes (20308 citations) PVDF-FL membranes (46 citations) PVDF-FL (6 citations)

2 protocols using immobilin fl pvdf membranes

SDS-PAGE and Western Blotting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

SDS-PAGE gels (4–15%) were run using the BioRad Mini-PROTEAN gel system. Gels were transferred onto Immobilin-FL PVDF membranes (Sigma) using the BioRad Trans-Blot SD Semi-Dry Transfer Cell. Membranes were blocked in 1% cold water fish skin gelatin (Sigma), then incubated with primary antibody at these ratios: anti-FLAG (1:2500, Sigma F3165), anti-biotin (1:1000, Jackson 200–002-211), anti-OGT (1:1000, Santa Cruz sc-74546), anti-β-actin (WB 1:2000, Cell Signaling 4970) Histone H3 (1:1000, Cell Signaling 14269), anti-GAPDH (WB, Cell Signaling 2118). Secondary antibodies were Li-Cor IRDye: 680RD donkey-anti mouse 680 (1:10000), 800CW Goat anti-rabbit (1:20000). Three washes in TBST (0.1% tween) were performed after each antibody incubation. All Western blots were imaged on a LiCor Odyssey Clx system. Densitometric measurements were made using Image Studio Lite v5.2

Stephen H.M., Praissman J.L, & Wells L. (2020). Generation of an Interactome for the Tetratricopeptide Repeat Domain of O-GlcNAc Transferase Indicates a Role for the Enzyme in Intellectual Disability. Journal of proteome research, 20(2), 1229-1242.

+ Open protocol

+ Expand

SDS-PAGE, Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

SDS-PAGE gels (4-15%) were run using the BioRad Mini-PROTEAN gel system. Gels were transferred onto Immobilin-FL PVDF membranes (Sigma) using the BioRad Trans-Blot SD Semi-Dry Transfer Cell. Membranes were blocked in 1% cold water fish skin gelatin (Sigma), then incubated with primary antibody at these ratios: anti-FLAG (1:2500, Sigma F3165), anti-biotin (1:10000), 800CW Goat anti-rabbit (1:20000). Three washes in TBST (0.1% tween) were performed after each antibody incubation. All Western blots were imaged on a LiCor Odyssey Clx system. Densitometric measurements were made using Image Studio Lite v5.2

Stephen H., Praissman J.L., & Wells L. (2020). Generation of an Unbiased Interactome for the Tetratricopeptide Repeat Domain of O-GlcNAc Transferase Indicates a Role for the Enzyme in Intellectual Disability.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!