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Hdr plasmid systems

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The HDR Plasmid Systems from Santa Cruz Biotechnology are a collection of plasmid vectors designed for efficient homology-directed repair (HDR) applications. These plasmids serve as templates for the HDR process, facilitating targeted gene insertions, deletions, or modifications in various cell lines and organisms.

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HDR plasmid (4 citations)

2 protocols using hdr plasmid systems

1

CD20 Knockout in JeKo-1 Cells

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FACS-sorted, stable JeKo-1-CD20-KO were generated using CD20-CRISPR/Cas9 and HDR Plasmid Systems (Santa Cruz Biotechnology, Santa Cruz, CA) according to manufacturer’s directions. CD20 knock-out was verified by flow cytometry and Western blots.
Qin H., Wei G., Sakamaki I., Dong Z., Cheng W.A., Smith D.L., Wen F., Sun H., Kim K., Cha S., Bover L., Neelapu S.S, & Kwak L.W. (2017). Novel BAFF-receptor antibody to natively folded recombinant protein eliminates drug resistant human B-cell malignancies in vivo. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(5), 1114-1123.
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2

CRISPR-Mediated CD19 Knockout in B-cell Lines

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FACS-sorted, stable Z-138-CD19-KO and Nalm-6-CD19-KO were generated using CD19-CRISPR/Cas9 and RFP reporter gene containing homologous directed repair (HDR) Plasmid Systems (Santa Cruz Biotechnology) according to the manufacturer’s directions. Briefly, DNA-In Transfection Reagent (MTI-GlobalStem) was used to transfect the plasmid system. RFP-positive cells were sorted by FACS and expanded. A stable CD19-deficient clone was verified by flow cytometry and Western blots for CD19 knock-out prior to banking for subsequent studies.
Qin H., Dong Z., Wang X., Cheng W.A., Wen F., Xue W., Sun H., Walter M., Wei G., Smith D.L., Sun X., Fei F., Xie J., Panagopoulou T.I., Chen C.W., Song J.Y., Aldoss I., Kayembe C., Sarno L., Müschen M., Inghirami G.G., Forman S.J, & Kwak L.W. (2019). CAR T cells targeting BAFF-R can overcome CD19 antigen loss in B cell malignancies. Science translational medicine, 11(511), eaaw9414.
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