The human pancreatic cancer cell lines (PANC‐1, SW1990, COLO357 and CF‐PAC1) and the human pancreatic ductal cell line (HPDE) were purchased from GeneChem (Shanghai, China). Cells were cultured in DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) which was supplemented with 10% foetal bovine serum (Gibco, Thermo Fisher Scientific) and 1% penicillin and streptomycin (Solarbio, Beijing, China), and they were maintained in a 37℃ incubator containing 5% CO2. The medium was replaced every 24‐48 hours according to the cell density. Cells were observed under an inverted microscope and were digested with 0.25% trypsin (Gibco, Thermo Fisher Scientific) to enable passaging of the cells when they reached 80% confluence.
Cf pac1
Manufactured by Genechem
Sourced in China
The CF-PAC1 is a lab equipment product from Genechem. It is a centrifugal particle analyzer designed to measure the size distribution of particles suspended in a liquid medium.
Automatically generated - may contain errors
Lab products found in correlation
Panc 1, by Genechem (4 mentions)
Sw1990, by Genechem (3 mentions)
Trypsin, by Thermo Fisher Scientific (2 mentions)
Colo357, by Genechem (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Penicillin and streptomycin, by Solarbio (2 mentions)
Bxpc 3, by Genechem (2 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Aspc 1, by Genechem (1 mentions)
M mlv, by Promega (1 mentions)
Mia paca 2, by Genechem (1 mentions)
Lightcycler 480 2, by Roche (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
4 protocols using cf pac1
1
Pancreatic Cancer Cell Line Cultivation
2
Cultivation of Pancreatic Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human normal pancreatic ductal epithelial cell line (HPDE6‐C7) was purchased from Genio Biotechnology Co., Ltd. The human PDAC cell lines AsPC‐1, BxPC‐3, CFPAC‐1, PANC‐1, and SW1990 were purchased from Genechem Co., Ltd. These cells were cultured in complete growth medium (Gibco) containing 10% fetal bovine serum (HyClone) under humidified conditions with 95% air, 5% CO2 at 37°C according to the manufacturer's instruction.
3
Quantifying ITGB6 Expression in Pancreatic Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of six pancreatic cancer cell lines (BXPC-3, CFPAC-1, MIA PaCa-2, ASPC-1, PANC-1 and SW1990) were provided by Shanghai GeneChem Co., Ltd. and cultured in RPMI-1640 basic medium (Corning, Inc.). All cells were routinely subcultured at 37˚C in the presence of 5% CO2 in an incubator with saturated humidity. Total RNA was extracted from the six cell lines using TRIzol reagent according to the manufacturer's instructions. RNA was reverse transcribed to complementary DNA using Promega M-MLV at 42˚C (Promega Corp.). The mRNA expression levels of the ITGB6 gene in different cell lines of interest were detected by quantitative PCR using a LightCycler 480 II (Roche Molecular Systems, Inc.). The composition of the reaction mixture was SYBR premix ex taq 6.0 µl, primer mix 0.3 µl, reverse transcription product 0.6 µl and RNase-free H2O 5.1 µl. The reaction conditions were as follows pre-denaturation at 95˚C for 30 sec, followed by denaturation for 5 sec at 95˚C and annealing for 30 sec at 60˚Cfor a total of 40 cycles. The primer sequences were as follows: ITGB6 forward, 5'-TGATCTTCGCTGTAACCC-3' and reverse, 5'-CAGACCGCAGTTCTTCATA-3'; GAPDH forward, 5'-TGACTTCAACAGCGACACCCA-3' and reverse, 5'-CACCCTGTTGCTGTAGCCAAA-3'. The experimental results were analyzed by the 2-∆∆Cq method (23 (link)) for relative quantitative analysis.
4
Cell Culture of Pancreatic Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human pancreatic cancer cell lines (PANC-1, SW1990, COLO357 and CF-PAC1) and the human pancreatic ductal cell line (HPDE) were purchased from GeneChem (Shanghai, China). Cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) was supplemented with 10% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin and streptomycin (Solarbio, Beijing, China), and they were maintained in a 37 incubator containing 5% CO 2 . The medium was replaced every 24-48 h according to the cell density. Cells were observed under an inverted microscope and were digested with 0.25% trypsin (Gibco, Thermo Fisher Scientific, Waltham, MA,USA) to enable passaging of the cells when they reached 80% confluence.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!