Log In Sign up
The largest database of trusted experimental protocols

Anti influenza a virus

Manufactured by Abcam
Visit Supplier
Sourced in United States

Anti-Influenza A virus is a laboratory product used to detect the presence of Influenza A virus in samples. It functions as an analytical tool to identify and quantify Influenza A virus.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd16 32 fc block, by BioLegend (2 mentions) Anti cd8α, by BD (2 mentions) Cm3050s cryostat, by BioLegend (2 mentions) Imaris software version 9, by Oxford Instruments (2 mentions) Anti epcam clone g8, by Thermo Fisher Scientific (2 mentions) Lsm 880 confocal microscope, by Zeiss (2 mentions) Anti cd11c clone n418, by BioLegend (2 mentions) Anti cd19 antibody, by Cell Signaling Technology (1 mentions) Anti ly6g antibody, by Abcam (1 mentions)

3 protocols using anti influenza a virus

1

Immunohistochemistry of Influenza A Virus

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were processed as previously described51 . Briefly, tissues were fixed in paraformaldehyde (PFA), lysine, and sodium periodate buffer (PLP, 0.05M phosphate buffer, 0.1M L-lysine, pH 7.4, 2 mg/mL NaIO4, and 10mg/mL PFA) overnight at 4°C followed by 30% sucrose overnight at 4oC and subsequent embedding in OCT media. Frozen tissues were sectioned at 20μm using Leica CM3050S cryostat, and FcR blocked with anti-CD16/32 Fc-block (Clone 93, BioLegend) diluted in PBS containing 2% donkey serum, 2% fetal bovine serum (FBS), and 0.1% Triton-X for 1h at 25oC. Sections were stained with anti-CD8α (Clone 53–6.7, BD Bioscience), anti-EpCAM (clone G8.8, eBioscience), anti-Influenza A virus (polyclonal, Abcam) and anti-CD11c (clone N418, BioLegend) diluted in PBS containing 2% goat serum, 2% FBS, 0.1% Triton-X, and 0.05% Fc block for 1h at 25oC. Images were acquired using a Zeiss LSM 880 confocal microscope (Carl Zeiss) with the Zen Black software. The imaging data were processed and analyzed using Imaris software version 9.0.1 (Bitplane, Oxford Instruments).
Barbet G., Nair-Gupta P., Schotsaert M., Yeung S.T., Moretti J., Seyffer F., Metreveli G., Gardner T., Choi A., Tortorella D., Tampé R., Khanna K.M., García-Sastre A, & Blander J.M. (2021). TAP dysfunction in dendritic cells enables non-canonical cross-presentation for T cell priming. Nature immunology, 22(4), 497-509.
+ Open protocol
+ Expand
2

Immunohistochemistry of Influenza A Virus

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were processed as previously described51 . Briefly, tissues were fixed in paraformaldehyde (PFA), lysine, and sodium periodate buffer (PLP, 0.05M phosphate buffer, 0.1M L-lysine, pH 7.4, 2 mg/mL NaIO4, and 10mg/mL PFA) overnight at 4°C followed by 30% sucrose overnight at 4oC and subsequent embedding in OCT media. Frozen tissues were sectioned at 20μm using Leica CM3050S cryostat, and FcR blocked with anti-CD16/32 Fc-block (Clone 93, BioLegend) diluted in PBS containing 2% donkey serum, 2% fetal bovine serum (FBS), and 0.1% Triton-X for 1h at 25oC. Sections were stained with anti-CD8α (Clone 53–6.7, BD Bioscience), anti-EpCAM (clone G8.8, eBioscience), anti-Influenza A virus (polyclonal, Abcam) and anti-CD11c (clone N418, BioLegend) diluted in PBS containing 2% goat serum, 2% FBS, 0.1% Triton-X, and 0.05% Fc block for 1h at 25oC. Images were acquired using a Zeiss LSM 880 confocal microscope (Carl Zeiss) with the Zen Black software. The imaging data were processed and analyzed using Imaris software version 9.0.1 (Bitplane, Oxford Instruments).
Barbet G., Nair-Gupta P., Schotsaert M., Yeung S.T., Moretti J., Seyffer F., Metreveli G., Gardner T., Choi A., Tortorella D., Tampé R., Khanna K.M., García-Sastre A, & Blander J.M. (2021). TAP dysfunction in dendritic cells enables non-canonical cross-presentation for T cell priming. Nature immunology, 22(4), 497-509.
+ Open protocol
+ Expand
3

Histopathology and Immunohistochemistry in Influenza

Check if the same lab product or an alternative is used in the 5 most similar protocols
NBF-fixed mouse and ferret lungs were processed for histopathology and immunohistochemistry as previously described (13) . Hematoxylin and eosin (H&E)-stained slides were examined from two mice or four ferrets per virus group at 5 days post-infection.
Immunohistochemistry was done on the same sets of fixed tissues as the histopathology. For the mouse lung tissues, Influenza A virus and immune cell (neutrophil, B and T cells) distribution were measured by immunohistochemistry. A goat polyclonal primary anti-influenza A virus (catalog no. ab20841; Abcam, USA) was used to stain influenza NP proteins. Anti-CD19 antibody (catalog no. 90176S; Cell Signaling Technology, USA), anti-CD3 antibody (catalog no. ab16669;
105 and is also made available for use under a CC0 license.
Abcam), and anti-Ly6G antibody (catalog no. ab210204; Abcam) were used to stain B cells, T cells, and neutrophils, respectively. For the ferret lung tissues, only Influenza A virus distribution was measured by immunohistochemistry. All slides were scanned on an Aperio ScanScope XT system (Aperio, USA), enabling whole-slide analysis.
Park J., Fong Legaspi S.L., Schwartzman L.M., Gygli S.M., Sheng Z.M., Freeman A.D., Matthews L.M., Xiao Y., Ramuta M.D., Batchenkova N.A., Qi L., Rosas L.A., Williams S.L., Scherler K., Gouzoulis M., Bellayr I., Morens D.M., Walters K.A., Memoli M.J., Kash J.C, & Taubenberger J.K. (2022). An inactivated multivalent influenza A virus vaccine is broadly protective in mice and ferrets. Science translational medicine, 14(653).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!