Cd103 clone m290

Manufactured by BD

The CD103 (clone M290) is a laboratory reagent used in flow cytometry applications. It is a monoclonal antibody that binds to the CD103 antigen, which is expressed on the surface of certain cell types. This product can be used to identify and quantify CD103-positive cells in biological samples.

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Lab products found in correlation

Cd11c clone n418, by Thermo Fisher Scientific (2 mentions) Cd11b clone m1 70, by BD (2 mentions) B220 clone ra3 6b2, by Thermo Fisher Scientific (1 mentions) Il 10, by BD (1 mentions) I a 1 e clone m5 114.15.2, by BioLegend (1 mentions) Cd3 clone 17a2, by BD (1 mentions) Mcp 1, by BD (1 mentions) Al oh 3, by Thermo Fisher Scientific (1 mentions) Cd40 clone 3 23, by BD (1 mentions) Ifn γ, by BD (1 mentions) Lps e coli 0111 b4, by Merck Group (1 mentions) Nk1.1 clone pk136, by BD (1 mentions) Gata3 clone l50 823, by BD (1 mentions) Ccl20, by R&D Systems (1 mentions) Cd45 clone 30 f11, by BioLegend (1 mentions) Gm csf, by R&D Systems (1 mentions) Lsrii fortessa, by BD (1 mentions) Collagenase 4, by Thermo Fisher Scientific (1 mentions) F4 80 clone bm8, by Thermo Fisher Scientific (1 mentions) Ova af647, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD103 (26 citations) CD103 (M290) (15 citations)

5 protocols using cd103 clone m290

Function-blocking Antibodies in Tumor Engraftment

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Function-blocking antibodies against mouse very late antigen (VLA)-1 (clone Ha31/8) and CD103 (clone M290) or isotype control antibodies were purchased in “No Azide, Low Endotoxin” format from BD (VLA-1) and Bioxcell (CD103). Two hundred fifty microgram was injected intraperitoneally (i.p.) on days 6, 11, and 16 following tumor engraftment.

Murray T., Fuertes Marraco S.A., Baumgaertner P., Bordry N., Cagnon L., Donda A., Romero P., Verdeil G, & Speiser D.E. (2016). Very Late Antigen-1 Marks Functional Tumor-Resident CD8 T Cells and Correlates with Survival of Melanoma Patients. Frontiers in Immunology, 7, 573.

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Allergen Extract Sensitization Protocol

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HDM extract was obtained from the Greer laboratories. The allergens were extracted from whole body crust of Dermatophagoides pteronyssinus, which contained 100 EU endotoxin/mg. E. coli 0111:B4 LPS was obtained from Sigma. Low endotoxin OVA (endotoxin level <1 EU/mg) was from Chondrex, Inc. Alum, which contained 40 mg/ml Al(OH)₃, was from Thermo-Fisher. Anti-mouse antibodies used for flow cytometry were CD11c (clone N418, eBioscience), I-A/I-E (Clone M5/114.152, Biolegend), CD11b (clone M1/70, BD), CD103 (clone M290, BD), CD64 (clone X54-5/7.1, BD), CD86 (clone GC1, BD), CD40 (clone 3/23, BD), CD45 (clone 30-F11, Biolegend), CD3 (clone 17A2, BD), B220 (clone RA3-6B2, eBioscience), NK1.1 (clone PK136, BD), GATA3 (clone L50-823, BD), and CD326 (clone G8.8, Biolegend). ELISA kits IL-4, IL-5, IL-10, IFN-γ, and MCP-1 were from BD; IL-13, KC, CCL20, GM-CSF, and IL-17 kits were from R&D Systems.

Qian G., Jiang W., Zou B., Feng J., Cheng X., Gu J., Chu T., Niu C., He R., Chu Y, & Lu M. (2018). LPS inactivation by a host lipase allows lung epithelial cell sensitization for allergic asthma. The Journal of Experimental Medicine, 215(9), 2397-2412.

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Multiparametric Flow Cytometry Analysis of Tumor Microenvironment

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Antibodies against mouse Ly6c (clone HK1.4), CD11b (clone M1/70), CD11c (clone N418), F4/80 (clone BM8), MHC-II (clone M5/114.15.2), CD45 (clone 30-F11), CD3 (clone 17A2), CD4 (clone GK1.5), CD4(clone H129.19), CD8 (clone 53–6.7), NK1.1 (clone PK136), NK1.1 (clone S17016D) CD45.2 (clone 104), CD24 (clone 30-F1), XCR1 (clone ZET), CD64 (clone X54–5/7.1), Thy1.1 (clone Ox-7), Th1.2 (clone 30-H12), CD69 (clone H1.2F3), CD31 (clone MEC13.3) were from Biolegend. Antibodies against mouse Ly6g (clone 1A8), CD16/32 (clone 2.4G2) and CD103 (clone M290) were from BD Biosciences. All of the antibodies from Biolegend and BD Biosciences are diluted as 1:100. Antibodies against mouse Calreticulin (Cat. No. ab196159) were from Abcam (diluted as 1:300). The live/dead dye Aqua (Cat. No. L34966) was from ThermoFisher (diluted as 1:300).
Tumor-bearing mice were sacrificed and the tumors were sliced and digested by collagenase IV (1 mg/ml, Thermo Scientific Cat. 17104019) for one hour at 37°C, and tumor draining lymph nodes were ground to make single cell suspensions. The single cells suspensions were filtered by 70 μm nylon strainers and stained as described^{45 (link)}. Stained samples were analyzed using a FACS analyzer (LSR-II or LSR-II-Fortessa) from BD Biosciences. All flow cytometry data were analyzed using FlowJo software (Flowjo LLC).

Li Y., Su Z., Zhao W., Zhang X., Momin N., Zhang C., Wittrup K.D., Dong Y., Irvine D.J, & Weiss R. (2020). Multifunctional oncolytic nanoparticles deliver self-replicating IL-12 RNA to eliminate established tumors and prime systemic immunity. Nature cancer, 1(9), 882-893.

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Multicolor Flow Cytometry Immune Profiling

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Isolated cells were treated with 2% FBS/PBS containing Fc block (BD Biosciences) for 20 min at 4°C and then stained with fluorescent-conjugated antibody. Antibodies used for flow cytometry analysis are as follows: CD11c (clone N418; eBiosciences), CD64 (clone X54-5/7.1; Biolegend), CD103 (clone M290; BD Biosciences), F4/80 (clone BM8; eBiosciences), CD11b (clone M1/70; BD Biosciences), CX₃CR1 (clone SA011F11; Biolegend), CD4 (clone RM4-5; eBiosciences), CD8α (clone 53–6.7; BD Biosciences), OVA-AF647 (Molecular Probe). Data were obtained using FACSCanto (BD Biosciences) and analyzed with FlowJo software (Tree Star, Ashland, OR).

Kim Y.I., Song J.H., Ko H.J., Kweon M.N., Kang C.Y., Reinecker H.C, & Chang S.Y. (2018). CX3CR1+ macrophages and CD8+ T cells control intestinal IgA production. Journal of immunology (Baltimore, Md. : 1950), 201(4), 1287-1294.

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Multiparameter Flow Cytometry Assay

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Flow cytometry analysis was performed using a FACS flow cytometer Fortessa (BD Biosciences). The data were analyzed using Flowjo software. CD45 (Clone 30-F11), CD4 (Clone GK1.5), CD8 (Clone 53–6.7), CD103 (Clone M290) and CD69 (Clone H1.2F3) were purchased from BD Bioscience. PD-1 (Clone 29F.1A12), Granzyme B (Clone GB11) and Foxp3 (Clone MF-14) were purchased from BioLegend. Ki-67 (Clone SolA15) and IFN-γ (Clone XMG1.2) were purchased from eBioscience (Thermo Fisher Scientific). For intracellular cytokine staining, harvested cells were stimulated with PMA (10 ng/ml) (catalogue number p1585–1G, Sigma) and ionomycin (1 μg/ml) (I9657–1G, Sigma) for 4h and incubated for the last 3h with brefeldin A (10 μg/ml) (catalogue number 00–4506-51, Thermo Fisher). The cells were transferred to a V-bottom plate, stained with surface marker antibodies in HBSS containing 1% FCS, fixed with 2% formaldehyde, and permeabilized with 0.5% saponin. The cells were stained with anti-IFN-γ and examined by flow cytometry.

Chen L., Sun R., Xu J., Zhai W., Zhang D., Yang M., Yue C., Chen Y., Li S., Turnquist H., Jiang J, & Lu B. (2020). Tumor-derived interleukin-33 promotes tissue-resident CD8+ T cells and is required for checkpoint blockade tumor immunotherapy. Cancer immunology research, 8(11), 1381-1392.

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