The apoptosis of HepG2 and HCCLM3 cells was measured using the TransDetect® Annexin V-FITC/PI Kit (FA101-01; TransGen Biotech Co., Ltd.) [27 (link)]. Briefly, 5 μl Annexin V-FITC was added to a 6-well plate, and the cells were incubated for 15 min in the dark at room temperature. The apoptosis rates of HCC cells were determined using a NovoCyte Advanteon B4 Flow Cytometer and NovoSampler Q software (Agilent Technologies Co., Ltd.).
Transdetect annexin 5 fitc pi kit
Manufactured by Transgene
Sourced in China
The TransDetect® Annexin V-FITC/PI Kit is a laboratory assay designed to detect and quantify apoptosis in cell samples. The kit utilizes two fluorescent dyes, Annexin V-FITC and Propidium Iodide (PI), to identify different stages of the apoptotic process. The core function of this product is to provide researchers with a tool to analyze and measure cell death and apoptosis within their experimental models.
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3 protocols using transdetect annexin 5 fitc pi kit
1
Apoptosis Measurement of HCC Cells
2
Annexin V-FITC/PI Assay of HAEC Pyroptosis
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The pyroptosis of HAECs was determined using the TransDetect® Annexin V-FITC/PI Kit (FA101-01; TransGen Biotech Co., Ltd., Beijing, China). Annexin V-FITC (5 μL) was added to HAECs seeded in 6-well cell culture plates and incubated at room temperature for 15 min in the dark. NovoCyte Advanteon B4 Flow Cytometer and NovoSampler Q software (Agilent Technologies Co., Ltd., CA, USA) were used for flow cytometry data acquisition and analyses.
3
Annexin V-FITC/PI Apoptosis Assay
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The TransDetect® Annexin V-FITC/PI Kit (FA101-01; TransGen Biotech Co., Ltd.) was used to measure the apoptosis of UM1 and HSC-2 cells according to a previous study [28 (link)]. Briefly, 5 μL Annexin V-FITC was added to a 6-well plate, and the cells were cultured for 15 min in the dark at room temperature. The apoptosis rates of OSCC cells were determined using a NovoCyte Advanteon B4 Flow Cytometer and NovoSampler Q software (Agilent Technologies Co., Ltd.).
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