Sc 21760

Manufactured by Santa Cruz Biotechnology

Sc-21760 is a laboratory product developed by Santa Cruz Biotechnology. It is a tool used for research purposes. The core function of this product is to facilitate scientific investigations, but its specific intended use should not be extrapolated.

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Lab products found in correlation

Erk1 2, by Cell Signaling Technology (1 mentions) Tween 20, by Merck Group (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Occludin, by Santa Cruz Biotechnology (1 mentions) Western lightning plus ecl enhanced chemiluminescence detection kit, by PerkinElmer (1 mentions) Phospho stat3, by Cell Signaling Technology (1 mentions) P nf κb, by Abcam (1 mentions) Occludin, by Abcam (1 mentions) Ab205587, by Abcam (1 mentions) Ab167161, by Abcam (1 mentions) Tnf α, by Abcam (1 mentions) Sc 722, by Abcam (1 mentions) Sc 293072, by Santa Cruz Biotechnology (1 mentions)

2 protocols using sc 21760

Western Blot Analysis of Inflammatory Signaling

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IB analysis was performed on tissue or cell lysates that were SDS-PAGE separated and transferred to nitrocellulose membranes. Blots were incubated with 5% (wt:vol) nonfat dry milk in PBS with 0.05% (wt:vol) Tween 20 (Millipore Sigma) (PBST) at room temperature for 1 hour to block nonspecific binding, and overnight at 4° C with primary antibodies in 3% BSA (wt:vol) in PBST and finally with HRP-conjugated secondary antibody in blocking buffer. Blots were developed using the Western Lightning Plus-ECL enhanced chemiluminescence detection kit (PerkinElmer). Immunoblotting was performed with antibodies against TLR2 (Santa Cruz, sc-21760), TLR4 (Santa Cruz, sc-293072), MyD88 (Santa Cruz, sc-74532), phospho-STAT3 (Cell Signaling, CS9145), STAT3 (Cell Signaling, CS12640), NLRP3 (LSBio, LS- C334192), FAS (Cell Signaling, #3180), ACC (Cell Signaling, #3662), ERK1/2 (Cell Signaling, #9102), Occludin (Santa Cruz, sc-271842) and Claudin 1 (Santa Cruz, sc- 166338). All primary antibodies were diluted 1:1000. Secondary antibodies were diluted 1:5000.

Todoric J., Caro G.D., Reibe S., Henstridge D.C., Green C.R., Vrbanac A., Ceteci F., Conche C., McNulty R., Shalapour S., Taniguchi K., Meikle P.J., Watrous J.D., Moranchel R., Najhawan M., Jain M., Liu X., Kisseleva T., Diaz-Meco M.T., Moscat J., Knight R., Greten F.R., Lau L.F., Metallo C.M., Febbraio M.A, & Karin M. (2020). Fructose stimulated de novo lipogenesis is promoted by inflammation. Nature metabolism, 2(10), 1034-1045.

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Protein Expression Analysis in Tissues

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Tissues were homogenized in RIPA buffer (150 mM sodium chloride, 1.0% NP-40 or Triton X-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulphate, 50 mM Tris, pH 8.0) (10% weight/volume). Tissue samples were separated on SDS-PAGE (10–12%), and transferred onto a polyvinylidene difluoride membrane. Blots were incubated with the following primary antibodies: BDNF (1:250), Abcam, Cat. No. ab203573, RRID: AB_2631315), TLR4 (1:1500), Santa Cruz, sc-293072, RRID: AB_10611320), TLR2 (1:1000), Santa Cruz, sc-21760, NRF2 (1:5000), Santa Cruz, sc-722, TNFα (1:10,000), Abcam, Cat No ab205587, occludin (1:50,000), Abcam, Cat. No ab167161, p-NF-κB (1:10,000), Abcam, Cat No ab86299. Membranes were washed with TBS-T (TBS + 0.05% Tween20), and then incubated with a secondary antibody linked to horseradish peroxidase. As a loading control, α-actin (1:1000) or GAPDH (1:50,000) was used. Immunoreactive bands were visualized using Chemidoc (Bio rad XRS + SYSTEM). The western blots were performed at least 3 times using independent blots. Densitometry analysis was performed using NIH Image J software. Values were normalized with actin or GAPDH and expressed as fold increase.

Syeda T., Sánchez-Tapia M., Orta I., Granados-Portillo O., Pérez-Jimenez L., Rodríguez-Callejas J.D., Toribio S., Silva-Lucero M.D., Rivera A.L., Tovar A.R., Torres N, & Perez-Cruz C. (2021). Bioactive Foods Decrease Liver and Brain Alterations Induced by a High-Fat-Sucrose Diet through Restoration of Gut Microbiota and Antioxidant Enzymes. Nutrients, 14(1), 22.

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