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4 protocols using ethyl phenylacetate

1

Authentic Standards for Flavor Analysis

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Authentic standards, including acetic acid, phenylacetaldehyde, β-damascenone, dimethyl disulfide, decanal, 4-ethyl-2-methoxyphenol, ethyl benzoate, ethyl lactate, ethyl 3-methylbutanoate, ethyl phenylacetate, furfuryl alcohol, 2-methoxyphenol, limonene, linalool, methional, methyl salicylate, 3-methylbutanal, nonanal, phenylethyl alcohol, sotolone, 3-isobutyl-2-methoxypyrazine (IBMP), 3-methylbutanoic acid and isoamyl acetate were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA) or Sigma-Aldrich China Co. (Shanghai, China).
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2

Volatile Ester Compound Isolation

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Ethyl acetate, ethyl 2-methylpropionate, propyl acetate, ethyl propionate, ethyl butyrate, isobutyl acetate, ethyl 2-methylbutyrate, ethyl 3-methyl butyrate, isoamyl acetate, ethyl valerate, butyl butyrate, ethyl hexanoate, isoamyl butyrate, propyl hexanoate, ethyl heptanoate, ethyl lactate, 3-methylbutyl hexanoate, propyl octanoate, ethyl nonanoate, hexyl hexanoate, ethyl 2-furoate, ethyl caprate, ethyl benzoate, ethyl phenylacetate, ethyl laurate, ethyl 3-phenylpropanoate, ethyl tetradecanoate, ethyl pentadecanoate, ethyl palmitate, ethyl oleate, and linoleic acid ethyl ester were obtained from Sigma-Aldrich (Shanghai, China). The internal standard (IS) was 2-octanol (Sigma-Aldrich, Shanghai, China). The linear retention index (RI) was determined with a C7–C30 n-alkane mixture (Sigma-Aldrich, Shanghai, China). All of the reagents used were of analytical grade with a purity of at least 97%, and most with a purity exceeding 99%. A Milli-Q purification system provided pure water (Millipore, Bedford, MA, USA). Sodium chloride (analytical grade) and absolute ethanol (analytical grade) were obtained from Sino-pharm Chemical Reagent Co., Ltd (Shanghai, China).
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3

Comprehensive Commercial Standards for Analysis

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A total of 66 commercial standards (analytical reagent grade, ≥97% purity) were used. Ethyl acetate, acetic acid, Ethyl lactate, 1-propanol, acetal, acetaldehyde, 3-methyl butanol, furfural, ethyl palmitate, isobutanol, methanol, 3-methylbutyraldehyde, ethyl butyrate, ethyl propionate, meso-2,3-butanediol, butyrate, 1-butanol, (2R,3R)-(-)-2,3-butanediol, 2-butanol, hexanoic acid, ethyl hexanoate, 3-hydroxy-2-butanone, ethyl isovalerate, furfuryl alcohol, isobutyric acid, hexyl hexanoate, ethyl valerate, 1-hexanol, propionic acid, acetone, ethyl laurate, 2-methylbutyraldehyde, isoamyl acetate, 2-butanone, ethyl isobutyrate, ethyl phenylacetate, nonanoic acid, propyl acetate, isobutyraldehyde, ethyl octanoate, 1,1-diethoxy-2-methylbutane, ethyl linoleate, valeric acid, ethyl oleate, ethyl heptanoate, ethyl formate, phenylethyl alcohol, ethyl hexadecenoate, 1-pentanol, ethyl tetradecanoate, diethyloxymethane, 2-pentanone, 2-methyl butanol, isovaleric acid, ethyl nonanoate, 2-pentanol, 1,1-diethoxy-3-methylbutane, benzaldehyde, ethyl decanoate, heptanoic acid, octanoic acid, 2-ethoxy-5-methylfuran, 1-propanal, butyl hexanoate, ethyl phenylpropionate, and benzyl alcohol were purchased from Sigma-Aldrich (Shanghai, China).
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4

Aroma Profiling of Model Wine Compounds

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The following chemical standards were used in the aroma base and were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA): ethanol (≥99%), 2,3-butanedione (97%), hexan-1-ol (98%), methionol (≥98%), butanoic acid (≥99%), decanoic acid (≥98%), 2-methylpropanoic acid (99%), 2-methylbutanoic acid (98%), 3-methylbutanoic acid (99%), 2-methylpropyl ethanoate (≥97%), ethyl phenylacetate (≥99%), ethyl 3-methylbutanoate (98%), ethyl 2-methylpropanoate ((≥98%), ethyl 2-methylbutyrate (99%), ethyl butanoate (≥98%), ethyl decanoate (≥99%), ethyl hexanoate (≥99%), ethyl octanoate (≥98%), hexanoic acid (99%), octanoic acid (≥99%), 2-methylpropan-1-ol (≥99%), ethyl acetate (≥99%) and isoamyl acetate (≥99%). Acetic acid (≥99%) was obtained from VWR International (Radnor, PA, USA). The terpenes compound added to the model wine for the treatments were: (S)-(−)-limonene (96%), (R)-(+)-limonene (97%), linalool (≥97%) and α-terpineol, which were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
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