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Methylcellulose culture medium

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Methylcellulose culture medium is a viscous, semi-solid media used for the culture and enumeration of hematopoietic stem and progenitor cells. It provides a three-dimensional, semisolid matrix that supports the growth and differentiation of these cells.

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Lab products found in correlation

Stem cell factor (scf), by BioLegend (1 mentions) Recombinant mouse il 7, by BioLegend (1 mentions)

Close spelling variants of this product

Methylcellulose (76 citations) Methylcellulose medium (29 citations) Methylcellulose culture (2 citations)

2 protocols using methylcellulose culture medium

1

Comprehensive Hematopoietic Stem Cell Assays

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Bulk or single cells were sorted into methylcellulose culture medium (Stemcell Technology). Myeloid colony-forming capacity was assessed by MethoCult™ GF M3534 for myeloid committed progenitors. HSC colony-forming capacity was tested by using MethoCult™ GF M3434. HSPC Pre-B colony formation was modified by supplementing MethoCult™ M3630 medium with recombinant mouse IL-7 (BioLegend) and SCF (BioLegend) as described previously.
Chen X., Deng H., Churchill M.J., Luchsinger L.L., Du X., Chu T.H., Friedman R.A., Middelhoff M., Ding H., Tailor Y.H., Wang A.L., Liu H., Niu Z., Wang H., Jiang Z., Renders S., Ho S.H., Shah S.V., Tishchenko P., Chang W., Swayne T.C., Munteanu L., Califano A., Takahashi R., Nagar K.K., Renz B.W., Worthley D.L., Westphalen C.B., Hayakawa Y., Asfaha S., Borot F., Lin C.S., Snoeck H.W., Mukherjee S, & Wang T.C. (2017). Bone marrow myeloid cells regulate myeloid-biased hematopoietic stem cells via a histamine-dependent feedback loop. Cell stem cell, 21(6), 747-760.e7.
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2

Multipotency of Hematopoietic Progenitors

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To investigate the multipotency of haematopoietic progenitor cells, colony-forming units were assayed using methylcellulose culture medium (Stem Cell Technologies, Canada). Aliquots of 1 × 105 cells were plated in 24-well plates and cultured for 14 days. Colony-forming unit erythroid (CFU-E), burst-forming unit erythroid (BFU-E), colony-forming unit granulocyte–macrophages (CFU-GMs), and colony-forming unit Mix (CFU-Mix) were counted. The cells in each group were seeded in triplicate.
Zhou S., Sun L., Qian S., Ma Y., Ma R., Dong Y., Shi Y., Jiang S., Ye H., Shen Z., Zhang S., Shen J., Yu K, & Wang S. (2021). Iron overload adversely effects bone marrow haematogenesis via SIRT-SOD2-mROS in a process ameliorated by curcumin. Cellular & Molecular Biology Letters, 26, 2.
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