All animal studies described in this manuscript have been approved by the IACUC at the University of Tennessee Health Science Center. All reagents were purchased from Sigma-Aldrich Chemical Co., Alfa Aesar (Ward Hill, MA), and AK Scientific (Mountain View, CA) and were used without further purification. Routine thin layer chromatography (TLC) was performed on aluminum-backed Uniplates (Analtech, Newark, DE). NMR spectra were obtained on a Varian Inova-500 spectrometer (Agilent Technologies, Santa Clara, CA) or a Bruker Ascend 400 (Billerica, MA) spectrometer. Chemical shifts are reported as parts per million (ppm) relative to TMS in CDCl3. High Resolution Mass spectra were collected in positive detection mode on a Waters Xevo G2-S Tof instrument equipped with an electron-spray ionization (ESI) source (Milford, MA).
Xevo g2 s tof
Manufactured by Waters Corporation
Sourced in United States
The Xevo® G2-S Tof is a high-resolution, time-of-flight mass spectrometer designed for advanced analytical applications. It offers high-performance mass analysis and separation capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Acquity uplc beh phenyl column, by Waters Corporation (4 mentions)
Acquity uplc system, by Waters Corporation (2 mentions)
Ascend 400, by Bruker (1 mentions)
Acquity ultra performance liquid chromatography system, by Waters Corporation (1 mentions)
Acquity beh c18 column, by Waters Corporation (1 mentions)
Oasis mcx 96 well plate, by Waters Corporation (1 mentions)
Spe c18 cartridge, by Phenomenex (1 mentions)
Strata c18 e, by Phenomenex (1 mentions)
H class uplc, by Waters Corporation (1 mentions)
Milli q, by Merck Group (1 mentions)
Integral 3 water purification system, by Merck Group (1 mentions)
Leucine enkephalin, by Waters Corporation (1 mentions)
Acquity uplc h class, by Waters Corporation (1 mentions)
9 protocols using xevo g2 s tof
1
Chemical Characterization of Compounds
2
LC/MS Protocol for CdG and ETV Analysis
3
Pharmacokinetics of CdG in ConA-induced VLI Rats
4
LC-MS Analysis of SK14-061a Compound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LC-MS was conducted on a Waters ACQUITY UPLC system with a TOF-MS system (Xevo® G2-S Tof, Waters). LC separations of SK14-061a were performed on a ACQUITY UPLC BEH Phenyl column (2.1 x 100 mm, 1.7 μm particle size, Waters) at 40°C. The mobile phases were an aqueous solution of 0.1% formic acid (solvent A) and 100% methanol (solvent B). The solvent gradient started at 95% A/5% B, changed to 5% A/95% B over 5 min after a 1 min hold. The gradient was returned to 95% A/5% B over 0.1 min and maintained for 4 min for the next run. The flow rate was 0.4 mL/min and the injection volume was 5 μl. The target mass for SK14-061a was 278.1.
5
Quantification of Diuron and Metabolites in Biofilms and Water
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diuron and its main metabolites (DCPMU and DCPU) in biofilms and water samples were quantified by UPLC-ToF (Ultra-Performance Liquid Chromatography -Time of Flight Mass Spectrometry, Xevo G2-S ToF, Waters) as proceeded in a previous study (see Chaumet et al., 2019 (link)). An ACQUITY BEH C18 column 50 × 2.1 mm, 1.7 μm (Waters) was used for chromatographic separation at 45°C with a binary gradient of ultrapure water (at pH= 5) and methanol (both solutions with ammonium acetate to a concentration of 10 mM, CAS: 631-61-8, ULC quality Biosolve), and 20 µL of samples were injected.
The gradient was performed with the following range: 98 % of ultra-pure water solution (0-3.6 min), 2 % of methanol solution (3.6-6 min) at a flow rate of 0.45 mL.min -1 .
Data were processed with MassLynx V4.1 software, and diuron concentrations were inferred via a calibration curve from 0 to 100 µg.L -1 , comprising 10 points. Every 10 samples, 3 quality controls were carried out at 0, 1 and 10 µg.L -1 on the calibration curve. For biofilm samples, data were normalized by dry weight and then expressed in µg.g -1 . Limits of quantification were established at 5 µg.L -1 for water samples and at 0.05 µg.mg -1 for biofilm samples.
The gradient was performed with the following range: 98 % of ultra-pure water solution (0-3.6 min), 2 % of methanol solution (3.6-6 min) at a flow rate of 0.45 mL.min -1 .
Data were processed with MassLynx V4.1 software, and diuron concentrations were inferred via a calibration curve from 0 to 100 µg.L -1 , comprising 10 points. Every 10 samples, 3 quality controls were carried out at 0, 1 and 10 µg.L -1 on the calibration curve. For biofilm samples, data were normalized by dry weight and then expressed in µg.g -1 . Limits of quantification were established at 5 µg.L -1 for water samples and at 0.05 µg.mg -1 for biofilm samples.
6
Quantitation of Cadmium-Guanine Conjugate
7
Biomarker Extraction and Identification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
During the extraction procedures, many tests were performed for each plant material to set the conditions able to extract the biomarkers compounds efficiently. Using an ultrasonic bath, the type of solvents or mixtures thereof, time of extraction, and liquid-to-solid ratio were modified in order to obtain the best conditions for each species. After the extraction, each extract was suspended at the proportion of 1mg/mL of solvent and cleaned up on SPE C18 cartridges (Strata-C18-E, 50 mg, 1 mL, Phenomenex, Torrance, CA, USA) previously conditioned with 1 mL ACN and subsequently 1 mL of water. Then, the cartridge was loaded with 1 mL of sample extract that was eluted twice with water/ACN (20:80), total volume being 1 mL.
For each species collected, a sample of plant material was taken before any treatment (referred to as step 0), and analyzed by LC-MS system (Xevo G2-SToF from Waters corp., Milford, MA, USA) to be used as a reference for each species. At this initial analytic step, some chemical constituents were identified and used as biomarkers for the present work. All samples were prepared and analyzed in triplicate. Aliquots of the treated samples were solubilized at a concentration of 1 mg/mL in water: ACN (20:80), filtered through a syringe filter (0.22 µm) and injected in an LC-MS instrument (5 µL).
For each species collected, a sample of plant material was taken before any treatment (referred to as step 0), and analyzed by LC-MS system (Xevo G2-SToF from Waters corp., Milford, MA, USA) to be used as a reference for each species. At this initial analytic step, some chemical constituents were identified and used as biomarkers for the present work. All samples were prepared and analyzed in triplicate. Aliquots of the treated samples were solubilized at a concentration of 1 mg/mL in water: ACN (20:80), filtered through a syringe filter (0.22 µm) and injected in an LC-MS instrument (5 µL).
8
Phytochemical Profiling of Cyperus rotundus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The main compounds of C. rotundus extracts were analyzed using ultra-high-performance liquid chromatography (Waters Acquity UPLC H-Class, Milford, MA, USA) tandem time-of-flight mass spectrometer (Waters Xevo G2-S tof, Milford, MA, USA) (UPLC-HRMS). The chromatographic column Acquity UPLC BEH C18, 2.1 mm × 50 mm, 1.7 µm was used to separate compounds. The column temperature was set at 45 • C, the injection volume was 1 µL, and the flow rate was 0.40 mL/min. The mobile phase comprised acetonitrile (A) and water containing 0.1% formic acid (B). The gradient elution procedures were as follows: 0-2 min, 3-10% (A); 2-6 min, 10-50% (A); 6-8 min, 50-80% (A); 8-11 min, 80-95% (A); 11-12 min, 95% (A); 12-13 min, 95-3% (A); 13-15 min, 3% (A). Electron spray ionization was used for the high-resolution mass spectrum (HRMS) in positive ion and negative ion mode, respectively. The temperature of the ion source was 120 • C. The other MS parameters were set as follows: the capillary voltage was 2.5 kV, the cone voltage was 25 V, the desolvation gas temperature was 400 • C, the desolvation gas flow was 1000 L/h, and the scanning pattern was MS E . The chemical information of different components was downloaded from the National Center for Biotechnology Information, the U.S. National Library of Medicine (https://pubchem.ncbi.nlm.nih.gov, Bethesda, MD, USA).
9
Trace Contaminant Analysis by UPLC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetonitrile (ACN) (HPLC plus gradient, LC-MS), water, methanol (MeOH) and formic acid (FA) (all LC-MS grade) were purchased from Carlo Erba. Ultrapure water (Milli-Q ® ) was produced by an Integral 3 water purification system from Millipore ® . The compound used for MS calibration was Leucine Enkephalin (LC-MS grade), purchased from Waters ® . Magnesium sulphate (MgSO 4 ) and sodium citrate (NaCit) salts (analytical grade) were provided by VWR France. Analyzes of trace contaminants have been performed on a Waters® Acquity H-Class UPLC ® system, composed of a quaternary solvent manager pump (QSM), a refrigerated sample manager Flow-Through-Needle (SM-FTN) and a column oven, coupled to a Waters ® high resolution mass spectrometer with a Time of Flight analyzer Xevo ® G2-S ToF (UHPLC/MS-ToF). An electrospray ionization source was used in both positive (ESI + ) and negative (ESI -) modes.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!