Sil 30ac

The level of jaspine B was measured using a validated LC-MS/MS method [7 (link)] in multiple reaction monitoring (MRM). The system was composed of liquid chromatography in tandem with mass spectrometry (Shimadzu, Columbia, MD, USA) with a controller (CBM-20A), two binary pumps (LC-30AD), an autosampler (SIL-30AC), and an AB Sciex (Foster City, CA, USA) QTRAP 5500 quadrupole mass spectrometer in positive electrospray ionization mode (ESI). The chromatograms were monitored and integrated by the Analyst 1.7.2 software (AB Sciex, Foster City, CA, USA).
LC separation was performed on an analytical reversed-phase column Kinetex^®C18 100 × 2.1 mm (1.7 μm) (Phenomenex, Torrance, CA, USA) by a combination of 0.1% formic acid in acetonitrile and water (85:15, v/v) in isocratic mode at a flow rate of 0.2 mL/min.
The positive ion ESI mass spectrometric parameters were as follows: capillary voltage; 5.5 kV, temperature; 250 °C, declustering potential (DP); 50 V, and collision cell exit potential (CXP); 15 V quantification was performed using MRM at m/z 299.8→270.1 (jaspine B), and m/z 336.1→320.0 (IS). Nitrogen was used as collision gas, and the collision energies were set at 30–40 eV. Calibration curves using peak height ratio (analyte over IS) were constructed over the range of 0.5 to 16 ng/mL in liposomes to measure jaspine B concentrations in liposomes.

The LC-MS/MS system comprised liquid chromatography (Shimadzu, MD, USA) with a binary pump (LC-30AD), an autosampler (SIL-30AC), a controller (CBM-20A) and an ABSciex QTRAP 5500 mass spectrometer (SCIEX, Redwood City, CA, USA). The chromatograms were monitored using Analyst 1.7, and the data were analyzed using MultiQuant 3.0 software (SCIEX, Redwood City, CA, USA). The metabolites were separated using Synergi™ Fusion-RP column (2.5 µm, 100 × 2 mm) obtained from Phenomenex (Torrance, CA, USA). The mass spectrometer was operated in negative electron spray ionization (ESI) mode, and the detection was performed in multiple reaction monitoring (MRM) mode. The summary of MS conditions precursor ions (Q1), product ions (Q3), declustering potential (DP), collision energy (CE), and ESI ion modes used for analysis are listed in Table 3.

LC-MS/MS system comprised of liquid chromatography (Shimadzu, MD, USA) with a binary pump (LC-30AD), an autosampler (SIL-30AC), a controller (CBM-20A), a degasser (DGU-20A5R), a column oven (CTO-20A), and an ABSciex QTRAP 5500 mass spectrometer (SCIEX, Foster City, CA, USA) with electron spray ionization (ESI) source. The chromatograms were monitored using Analyst 1.7 software, and the data were analyzed in MultiQuant 3.0 software (SCIEX, Foster City, CA, USA). The analytes were separated using Synergi™ Fusion-RP column (2.5 µm, 100 × 2 mm) obtained from Phenomenex (Torrance, CA, USA). All the analyses were performed in positive ion mode.