Ly 6g ly 6c 14 5931 82

Rehmanniae Radix and Ginseng Radix were supplied by Jiangsu Province Hospital on Integration of Chinese and Western Medicine (Nanjing, China). Poria was collected from Guizhou Province, the genuine producing area of Poria in China. All herb samples were authenticated according to the monographs documented in Chinese Pharmacopeia (part I, 2020 version). Cisplatin (H20023461) was purchased from Qilu pharmaceutical Co., Ltd. (Jinan, China). Antibiotics, including ampicillin (A70006), metronidazole (M80002), neomycin (N80003), and vancomycin (V10116), were provided by Beijing Jin Ming biotechnology Co., Ltd. (Beijing, China). TGF-β1 antibody (ab215715) was purchased from Abcam (Cambridge, UK). Antibodies, including Smad2/3 (8685), α-SMA (19245), PARP (9532), p53 (2524), histone H3 (4499), and Ac-H3 (9649), were purchased from Cell Signaling Technology (Beverly, MA, USA). F4/80 (14-4801-82) and Ly-6G/Ly-6C (14-5931-82) monoclonal antibodies were from Invitrogen (Carlsbad, CA, USA), and GAPDH (glyceraldehyde-3-phosphate dehydrogenase; 60004-1) was from Proteintech (Rosemont, IL, USA). Reference compounds, including acetic acid (A116165), propionic acid (P110443), butyric acid (B110439), and 2-methylvaleric acid (M117868), were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). All other reagents used were of at least analytical purity.

Rehmanniae Radix and Ginseng Radix were supplied by Jiangsu Province Hospital on Integration of Chinese and Western Medicine (Nanjing, China). Poria was collected from Guizhou Province, the genuine producing area of Poria in China. All herb samples were authenticated according to the monographs documented in Chinese Pharmacopeia (part I, 2020 version). Cisplatin (H20023461) was purchased from Qilu pharmaceutical Co., Ltd. (Jinan, China). Antibiotics, including ampicillin (A70006), metronidazole (M80002), neomycin (N80003), and vancomycin (V10116), were provided by Beijing Jin Ming biotechnology Co., Ltd. (Beijing, China). TGF-β1 antibody (ab215715) was purchased from Abcam (Cambridge, UK). Antibodies, including Smad2/3 (8685), α-SMA (19245), PARP (9532), p53 (2524), histone H3 (4499), and Ac-H3 (9649), were purchased from Cell Signaling Technology (Beverly, MA, USA). F4/80 (14-4801-82) and Ly-6G/Ly-6C (14-5931-82) monoclonal antibodies were from Invitrogen (Carlsbad, CA, USA), and GAPDH (glyceraldehyde-3-phosphate dehydrogenase; 60004-1) was from Proteintech (Rosemont, IL, USA). Reference compounds, including acetic acid (A116165), propionic acid (P110443), butyric acid (B110439), and 2-methylvaleric acid (M117868), were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). All other reagents used were of at least analytical purity.