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3 protocols using nb100 56708ss

1

Apoptosis and Autophagy Regulation in Cancer

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Antibodies used in these experiments included the following: anti‐cleaved‐caspase3 (ab32042, Abcam), anti‐caspase3 (NB100‐56708SS, Novus), anti‐caspase9(ab32539, Abcam), anti‐BAX (50599‐2‐Ig, Proteintech), anti‐Bcl‐2 (GTX100064, GeneTex) anti‐GAPDH (#5174, Cell Signaling Technology), anti‐P62 (M162‐3, Medical Biological Laboratories), anti‐Beclin1 (11306‐1‐AP, Proteintech), anti‐LC3B (GB11124, Servicebio), anti‐ATG16L1(#8089, Cell Signaling Technology), anti‐ATG7(#8558, Cell Signaling Technology), anti‐p53(SC‐126, Santa Cruze), and anti‐ubiquitin(10201‐2‐AP, Proteintech).
Reagents used in these experiments included the following: autophagy inhibitor 3‐Methyladenine (3‐MA) (S2767, Selleck), p53 pathway inhibitor Pifithrin‐α (PFT‐α) (S2929, Selleck), temozolomide (TMZ) (S1237, Selleck), MG132 (S2619, Selleck), and cycloheximide (CHX)(HY‐12320, MCE).
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2

Antibody Screening for Cell Signaling

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Antibodies used in these experiments included the following: anti-RND2 (13844–1-AP, Proteintech, USA), anti-Rho7/Rnd2 (GXT56070, GeneTex, USA), anti-p-p38 (#4511, Cell Signaling Technology, USA), anti-p38 (#9212, Cell Signaling Technology, USA), anti-cleaved-caspase3 (ab32042, Abcam, UK), anti-caspase3 (NB100-56708SS, Novus, USA), anti-BAX (50599–2-Ig, Proteintech, USA), anti-GAPDH (#5174, Cell Signaling Technology), anti-P62 (M162–3, Medical Biological Laboratories, Japan), anti-Beclin1 (11306–1-AP, Proteintech, USA), anti-LC3B (GB11124, Servicebio, China), anti-DYKDDDK/Flag-tag (ANT102, Antgene, China), and anti-His-tag (D291–3, Medical Biological Laboratories, Japan). The autophagy inhibitor wortmannin (3-MA) and the autophagy activator rapamycin (Sirolimus) (S1039, USA) were purchased from Selleck (S2758, USA).
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3

Antibodies for Autophagy Pathway Study

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Antibodies used in this experiment included the following items: anti-RND2 (13844-1-AP, Proteintech, USA), anti-Rho7 /Rnd2 (GXT56070, from GeneTex in USA), anti-p-p38(#4511, from Cell Signaling Technology in USA), anti-p38 (#9212, from Cell Signaling Technology in USA), anti-cleaved-caspase3 (ab32042, from Abcam in UK), anti-caspase3 (NB100-56708SS, from Novus in USA), anti-Bax (50599-2-Ig, from Proteintech in USA), anti-GAPDH (#5174, from Cell Signaling Technology), anti-SQSTM1/P62 (M162-3, from Medical Biological Laboratories in Japan), anti-Beclin1 (11306-1-AP, from Proteintech in USA), anti-LC3B (GB11124, from Servicebio in China), USA), anti-DYKDDDK/Flag-tag (ANT102, from Antgene in China), and anti-His-tag (D291-3, from Medical Biological Laboratories in Japan). Autophagy inhibitor Wortmannin (3-MA) and the autophagy activator Rapamycin (Sirolimus) (S1039, from USA) were bought from Selleck (S2758, USA).
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