The Abs used for immunoblotting were rabbit anti-PTBP1 Ab (382421, Zen Bioscience), rabbit anti-PSF Ab (15585-1-ap, Proteintech), rabbit anti-H3 Ab (17168-1-ap, Proteintech), mouse anti–hRNP L Ab (ab6106, Abcam), rabbit anti–hRNP U Ab (ab172608, Abcam), rabbit anti–hRNP A1 Ab (ab208026, Abcam), mouse anti–hRNP F Ab (04-1462, Sigma-Aldrich), and mouse anti-actin Ab (66009-1-Ig, Proteintech). The Abs used for co-IP were mouse anti-PTBP1 Ab (MABE986, Sigma-Aldrich) and mouse anti-PSF Ab (P2860, Sigma-Aldrich). The Abs used for RIP analysis were mouse anti-PTBP1 Ab (MABE986, Sigma-Aldrich), mouse anti-PSF Ab (P2860, Sigma-Aldrich), mouse anti–hRNP L Ab (ab6106, Abcam), rabbit anti–hRNP U Ab (ab172608, Abcam), rabbit anti–hRNP A1 Ab (ab208026, Abcam), mouse anti–hRNP F Ab (04-1462, Sigma-Aldrich), and mouse normal immunoglobulin G (IgG; sc-2025, Santa Cruz Biotechnology). The Abs used for IF were mouse anti-PTBP1 Ab (MABE986, Sigma-Aldrich), rabbit anti-PSF Ab (15585-1-AP, Proteintech), fluorescein isothiocyanate–conjugated donkey anti-mouse IgG (SA00003-9, Proteintech), and CoraLite 647–conjugated donkey anti-rabbit IgG (SA00014-7, Proteintech).
Miao H., Wu F., Li Y., Qin C., Zhao Y., Xie M., Dai H., Yao H., Cai H., Wang Q., Song X, & Li L. (2022). MALAT1 modulates alternative splicing by cooperating with the splicing factors PTBP1 and PSF. Science Advances, 8(51), eabq7289.
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