Gonggan (Citrus reticulata Blanco var. gonggan from Yulin City, Guangxi Zhuang Autonomous Region, China) peel was removed and crushed into powder after being freeze-dried in lyophilizer (Ningbo Scientz Biotechenology Co., Ltd. Ningbo City, China). One hundred grams of dried citrus peel powder was added to 80% ethanol with a liquid-to-material ratio of 20:1 and then heated at 80°C for 4 h (Sharma et al., 2019 (link)). AB-8 macroreticular resin (Beijing Solarbio Science and Technology Co., Ltd. Beijing, China) was used to purify the crude extract. The macroporous adsorption resin was eluted by 90% ethanol (5 BV) and the eluent rate was 15 ml/min (Nie et al., 2017 (link)). Ethanol eluent was combined and evaporated under reduced pressure. The residue was freeze-dried and then ground to produce powder of GPE. The total flavonoids of GPE were obtained by detecting the absorbance at the wavelength of 500 nm (Evolution 300 ultraviolet spectrophotometer, Thermo Fisher Scientific, Inc. Waltham, MA, United States) with rutin as the standard substance according to the literature (Liu et al., 2019 (link)).
Ab 8 macroreticular resin
Manufactured by Solarbio
Sourced in China
AB-8 macroreticular resin is a type of chromatographic resin. It is a porous polymer material with a large surface area and a macroreticular structure. The resin is designed for general-purpose separation and purification applications in laboratory settings.
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Lab products found in correlation
2 protocols using ab 8 macroreticular resin
1
Extraction and Quantification of Gonggan Citrus Flavonoids
2
Extraction and Quantification of Gonggan Peel Flavonoids
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The peel of gonggan (Citrus reticulata Blanco var. gonggan from Yulin City, Guangxi Zhuang Autonomous Region, China) was freeze-dried and ground into a powder. A solution of citrus peel powder (100 mg) and 80% ethanol solution with a liquid-to-material ratio of 20:1 was heated at 80°C for 4 h.20 (link),21 (link) To purify the crude extract, AB-8 macroreticular resin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) was used with a 90% ethanol eluent. When the eluent became colorless, the separation was stopped and the eluates were combined, and evaporated under reduced pressure. The residue was freeze-dried and then ground into powder to be used as the source of GPFE. The total flavonoids of GPFE were obtained by detecting the absorbance at the wavelength of 500nm (Evolution 300 ultraviolet spectrophotometer, Thermo Fisher Scientific, Inc., Waltham, MA, USA) with rutin as standard substance.
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