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Cmp6010

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The CMP6010 is a plant growth chamber designed by Conviron to provide a controlled environment for plant research and cultivation. It offers precise regulation of temperature, humidity, lighting, and other environmental parameters to create optimal conditions for plant growth and experimentation.

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Lab products found in correlation

Polyethylene glycol 8000, by Merck Group (2 mentions) Vapro osmometer, by Wescor (2 mentions)

11 protocols using cmp6010

1

Arabidopsis and Cotton Seed Germination and Salt Stress

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The seeds of Arabidopsis Columbia (Col-0) were suspended in 0.1% (w/v) agarose solution and sown in pots filled with growth medium consisting of perlite, vermiculite, and peat. The pots were placed in a growth chamber (Conviron CMP6010, Canada) at 22/20 °C, 16 h light/8 h dark (day/night) for germination and growth.
Seeds of three different cotton varieties, including G. hirsutum L. TM-1, G. arboreum L. Shixiya1, and G. raimondii, were collected from the germplasm bank of the state key laboratory of cotton biology of China. The seeds were soaked in sterile water for 4 h and allowed to germinate on wet filter paper at 25 °C for 1–2 days. The germinating seeds were planted in growth medium in a growth chamber at 25/22 °C and 16 h light/8 h dark (day/night). After 4 weeks, the well-grown plants were treated with water or salt. For salt treatment, each cotton seedling was watered with 50 mL of 300 mM NaCl solution. Cotton seedlings treated with deionized water were used as mock. For phenotypic observation, photographs were taken after 12 days of treatment. Salt ion concentration was detected after photographing.
Long L., Zhao J.R., Guo D.D., Ma X.N., Xu F.C., Yang W.W, & Gao W. (2020). Identification of NHXs in Gossypium species and the positive role of GhNHX1 in salt tolerance. BMC Plant Biology, 20, 147.
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2

Arabidopsis Growth Optimization Protocol

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Arabidopsis plants of wild type (ecotype Columbia-0 or Col-0), dorn1-3 (Choi et al., 2014 (link)), and transgenic lines were each surface- sterilized and sown on a solid medium containing half-strength Murashige and Skoog (MS) (Murashige and Skoog, 1962 (link)) with vitamins (MSP09; Casson, Smithfield, UT, USA), 1% (w/v) sucrose, and 1% (w/v) agar Daishin (RPI, Mt. Prospect, IL, USA) and buffered in 0.05% (w/v) MES-KOH (pH 5.7) in a square petri dish. Seeds were cold- stratified in the dark for 3 days at 4°C and grown vertically in a 22°C growth chamber (CMP6010; Conviron, Winnipeg, MB, Canada) with a 12-h photoperiod (~100 µmol photons m−2 s−1).
Sowders J.M, & Tanaka K. (2023). A histochemical reporter system to study extracellular ATP response in plants. Frontiers in Plant Science, 14, 1183335.
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3

Soil Inoculation with Metarhizium anisopliae

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After 19 days, individual containers containing fumigated, pasteurized soil or field soil received 5 mL of sterile water before being inoculated with M. anisopliae (CAGMa+Cs+By) (Figure 1A) or food (CAGCs+By) granules (Figure 1B). Prepared M. anisopliae granules were placed on the soil surface at a rate of 4 × 108 conidia g−1 soil equivalent to 1 g (~60 pieces) of granules. Food granules without conidia were inoculated into the respective containers with soil as the control treatment. A Petri dish containing 3% water agar was inoculated with either ten fungal granules or food granules for quality control (to assess for resporulation and the presence of contaminants), sealed with Parafilm® and incubated in a plant growth chamber (CONVIRON® CMP6010, Melbourne, Australia) at 25 °C, 80% RH and a 12/12 h day and night photoperiod. After inoculation, the containers were loosely sealed with a lid and transferred to the same plant growth chamber and incubated at 25 °C, 80% relative humidity (RH), with a 12:12 h dark and light photoperiod. There were six replicates per treatment and containers were arranged in a randomized complete block design (RCBD).
Shah S., Ash G.J, & Wilson B.A. (2022). Resporulation of Calcium Alginate Encapsulated Metarhizium anisopliae on Metham®-Fumigated Soil and Infectivity on Larvae of Tenebrio molitor. Journal of Fungi, 8(10), 1114.
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4

Arabidopsis Growth Conditions Optimization

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Arabidopsis seeds were surface sterilized (70% v/v EtOH for 5 min followed by 6% v/v hypochlorite for 2 min and six 1-min rinse steps with ddH2O) and germinated in half-strength Murashige and Skoog agar medium (MS, salts 0.215% w/v, 1% w/v sucrose, 1% w/v agar, pH 5.7) in vertical plates. Seedlings were transferred when 7-day-old to substrate (Sunshine Mix #3 plus 1:4 volume perlite:substrate, autoclaved for 2 h and mixed with 2% w/w slow liberation fertilizer NPK 12:12:17) and watered every 2 days. Germination and growth was under long-day conditions (16 h light/8 h dark, 150 µE m−2 s−1, 60% humidity) in a growth chamber (Conviron CMP6010). ZT0 (Zeitgeber Time) was the start of the light cycle (day). Genotypes were grown side-by-side in a randomized manner to minimize experimental noise.
Núñez-López L., Aguirre-Cruz A., Barrera-Figueroa B.E, & Peña-Castro J.M. (2015). Improvement of enzymatic saccharification yield in Arabidopsis thaliana by ectopic expression of the rice SUB1A-1 transcription factor. PeerJ, 3, e817.
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5

Micropropagation and Pathogen Assays of Piper spp.

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Cuttings of P. colubrinum were rooted in sterile soilrite mixture (peat moss:vermiculite:perlite, 1:1:1, v/v/v, Keltech Energies Ltd., Bangalore, India). Rooted plants of uniform age were maintained in the growth chamber (Conviron CMP6010) with the temperature adjusted to 24 °C and humidity of 70% and under a 16/8 h light/dark regime in the Plant Tissue Culture facility of Rajiv Gandhi Centre for Biotechnology. Young leaves from 2-month-old cuttings were used for RNA isolation and cloning of osmotin gene.
Cuttings of Piper nigrum were similarly maintained under controlled conditions in the growth chamber and young leaves from 3-month-old cuttings were selected for pathogen infection assays.
Geetha R.G., Krishnankutty Nair Chandrika S., Saraswathy G.G., Nair Sivakumari A, & Sakuntala M. (2021). ROS Dependent Antifungal and Anticancer Modulations of Piper colubrinum Osmotin. Molecules, 26(8), 2239.
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6

Arabidopsis and Cotton Cultivation and Salt Stress

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The seeds of Arabidopsis Columbia (Col-0) were suspended in 0.1% (w/v) agarose solution and sown in pots filled with growth medium consisting of perlite, vermiculite, and peat. The pots were placed in a growth chamber (Conviron CMP6010, Canada) at 22/20 °C, 16 h light/8 h dark (day/night) for germination and growth.
Seeds of three different cotton varieties, including G. hirsutum L. TM-1, G. arboreum L. Shixiya1, and G. raimondii, were collected from the germplasm bank of the state key laboratory of cotton biology of China. The seeds were soaked in sterile water for 4 h and allowed to germinate on wet filter paper at 25 °C for 1-2 days. The germinating seeds were planted in growth medium in a growth chamber at 25/22 °C and 16 h light/8 h dark (day/night). After 4 weeks, the well-grown plants were treated with water or salt. For salt treatment, each cotton seedling was watered with 50 mL of 300 mM NaCl solution. Cotton seedlings treated with deionized water were used as mock. For phenotypic observation, photographs were taken after 12 days of treatment. Salt ion concentration was detected after photographing.
Long L., Zhao J., Guo D., Bai L., Xu F., Yang W., & Gao W. (2020). Identification of NHXs in Gossypium species and the positive role of GhNHX1 in salt tolerance.
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7

Water Potential Effects on Amaranthus Germination

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Germination (both parental and progeny generations) was tested under five nominal water potentials (0, -0.2, -0.4, -0.6, and -0.8 MPa) at two constant temperatures of 20 and 30 C representing suboptimal and optimal temperatures for germination of A. palmeri, respectively (Guo and Al-Khatib 2003; Steckel et al. 2004) . Experiments were conducted with four replicates (petri dishes) per treatment and repeated twice. Experimental runs were rotated between two growth chambers (Conviron® CMP 6010, Winnipeg, Canada). Solutions were prepared by dissolving appropriate amounts of polyethylene glycol 8000 (Sigma-Aldrich, St. Louis, MO) in deionized water according to Michel (1983) . Filter papers (Whatman® Grade 2; Sigma-Aldrich) were soaked in each solution containing the desired water potential for 12 h before the experiment, as recommended by Hardegree and Emmerich (1990) . The water potential of each solution was tested twice: before and after adding the filter papers to the solution using a Wescor Vapro osmometer (Wescor, Logan, UT, USA). For each treatment, 40 seeds from each population were placed in a 9-cm petri dish lined with moistened filter paper and sealed with Parafilm® to reduce water loss from the dishes.
Matzrafi M., Osipitan O.A., Ohadi S, & Mesgaran M.B. (2021). Under Pressure: Maternal Effects Promote Drought Tolerance in Progeny Seed of Palmer Amaranth (Amaranthus palmeri). Weed Science., 69(1).
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8

Comparing Salt Tolerance in Cotton Varieties

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The seeds of Arabidopsis were suspended in 0.1% (w/v) agarose solution and sown in pots lled with growth medium consisting of perlite, vermiculite, and peat. The pots were placed in a growth chamber (Conviron CMP6010, Canada) at 22/20 °C, 16 h light/8 h dark (day/night) for germination and growth.
Seeds of three different cotton varieties, including G. hirsutum L. TM-1, G. arboreum L. Shixiya1, and G. raimondii, were collected from the germplasm bank of the state key laboratory of cotton biology of China. The seeds were soaked in sterile water for 4 h and allowed to germinate on wet lter paper at 25 °C for 1-2 days. The germinating seeds were planted in growth medium in a growth chamber at 25/22 °C and 16 h light/8 h dark (day/night). After 4 weeks, the well-grown plants were treated with water or salt. For salt treatment, each cotton seedling was watered with 50 mL of 300 mM NaCl solution. Cotton seedlings treated with deionized water were used as mock. For phenotypic observation, photographs were taken after 12 days of treatment. Salt ion concentration was detected after photographing.
Long L., Zhao J., Guo D., Bai L., Xu F., Yang W., & Gao W. (2020). Identification of NHXs in Gossypium species and the positive role of GhNHX1 in salt tolerance.
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9

Water Potential Effects on Amaranthus Germination

Check if the same lab product or an alternative is used in the 5 most similar protocols
Germination (both parental and progeny generations) was tested under five nominal water potentials (0, -0.2, -0.4, -0.6, and -0.8 MPa) at two constant temperatures of 20 and 30 C representing suboptimal and optimal temperatures for germination of A. palmeri, respectively (Guo and Al-Khatib 2003; Steckel et al. 2004) . Experiments were conducted with four replicates (petri dishes) per treatment and repeated twice. Experimental runs were rotated between two growth chambers (Conviron® CMP 6010, Winnipeg, Canada). Solutions were prepared by dissolving appropriate amounts of polyethylene glycol 8000 (Sigma-Aldrich, St. Louis, MO) in deionized water according to Michel (1983) . Filter papers (Whatman® Grade 2; Sigma-Aldrich) were soaked in each solution containing the desired water potential for 12 h before the experiment, as recommended by Hardegree and Emmerich (1990) . The water potential of each solution was tested twice: before and after adding the filter papers to the solution using a Wescor Vapro osmometer (Wescor, Logan, UT, USA). For each treatment, 40 seeds from each population were placed in a 9-cm petri dish lined with moistened filter paper and sealed with Parafilm® to reduce water loss from the dishes.
Matzrafi M., Osipitan O.A., Ohadi S., & Mesgaran M.B. (2020). Under pressure: maternal effects promote drought tolerance in progeny seed of Palmer amaranth (Amaranthus palmeri). Weed Science, 69(1), 31-38.
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10

Arabidopsis and Cotton Seed Germination

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The seeds of Arabidopsis were suspended in 0.1% (w/v) agarose solution and sown in pots filled with growth medium consisting of perlite, vermiculite, and peat. The pots were placed in a growth chamber (Conviron CMP6010, Canada) at 22/20 °C, 16 h light/8 h dark (day/night) for germination and growth.
Seeds of three different cotton varieties, including G. hirsutum L. TM-1, G. arboreum L. Shixiya1, and G. raimondii, were collected from the germplasm bank of the state key laboratory of cotton biology of China. The seeds were soaked in sterile water for 4 h and allowed to germinate on wet filter paper at 25 °C for 1-2 days. The germinating seeds were planted in growth medium in a growth chamber at 25/22 °C and 16 h light/8 h dark (day/night). After 4 weeks, the well-grown plants were treated with water or salt. For salt treatment, each cotton seedling was watered with 50 mL of 300 mM NaCl solution. Cotton seedlings treated with deionized water were used as mock. For phenotypic observation, photographs were taken after 12 days of treatment. Salt ion concentration was detected after photographing.
Long L., Zhao J., Guo D., Ma X., Xu F., Yang W., & Gao W. (2020). Identification of NHXs in Gossypium species and the positive role of GhNHX1 in salt tolerance.
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