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Primary antibodies against p mtor

Manufactured by Cell Signaling Technology

Primary antibodies against p-mTOR are laboratory reagents used to detect and quantify the phosphorylated form of the mammalian target of rapamycin (mTOR) protein. mTOR is a key regulator of cell growth, proliferation, and metabolism. These antibodies can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the activation state of the mTOR signaling pathway.

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2 protocols using primary antibodies against p mtor

1

Investigating PI3K/AKT/mTOR Signaling

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Gemcitabine and paclitaxel were purchased from MedChemExpress LLC. Protease inhibitor cocktail and phosphatase inhibitor cocktail were purchased from APExBIO Technology LLC. Primary antibodies against p-mTOR, mTOR, p-AKT, AKT, p-PI3K, PI3K, MDR1, cleaved caspase-3, Beclin-1, LC3A/B and GAPDH were purchased from Cell Signaling Technology, Inc.
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2

Polyphyllin I Induces Autophagy

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Polyphyllin I (PPI) was purchased from Chengdu Mansite Pharmacetical CO, LTD (Purity ≥ 98%, lot number: MUST—16021905, Chengdu, China), and L-15 medium was purchased from Hyclone (SH30525.01, Logan, UT, USA). FBS was purchased from Gibco (Waltham, MA, USA). DMSO, acridine orange (AO) staining kit and mounting medium were purchased from Solarbio (Beijing, China). MTT and CQ were purchased from Sigma (St Louis, MI, USA). Primary antibodies against β-actin, LC3/Atg8, and mTOR were purchased from Boster (Wuhan, China). Primary antibodies against p-mTOR, AKT, p-AKT, and p53 were purchased from Cell Signaling. ROS assay kits were purchased from Beyotime (Chengdu, China).
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