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Tnf alpha mouse proquantum immunoassay kit

Manufactured by Thermo Fisher Scientific
Sourced in United States

The TNF alpha Mouse ProQuantum Immunoassay Kit is a laboratory diagnostic tool used for the quantitative detection of tumor necrosis factor alpha (TNF-alpha) in mouse samples. This kit utilizes an enzyme-linked immunosorbent assay (ELISA) principle to measure the concentration of TNF-alpha.

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2 protocols using tnf alpha mouse proquantum immunoassay kit

1

Comprehensive Metabolic Profiling of Murine Plasma

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Plasma fatty acid, triglyceride, cholesterol, alanine transaminase and aspartate transaminase were analysed with the serum analyser (Beckman Coulter, AU480). Insulin levels were determined by using the Ultra-Sensitive Mouse Insulin ELISA Kit (CrystalChem, Zaandam, Netherlands) according to manufacturer’s instructions. Ketone body measurements were analysed in tail vein blood (Wellion, Marz, Austria). Plasma TNFα levels were analysed by using the TNF alpha Mouse ProQuantum Immunoassay Kit (Thermo Fisher Scientific, Waltham, MA, United States) according to the manufacturer’s instructions. Murine plasma proteome was analysed by using the Proteome Profiler Mouse Adipokine Array Kit (R&D systems, Minneapolis, Canada) according to the manuals.
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2

Quantifying Inflammatory Cytokines in AP Mice

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The cell supernatant or serum of AP mice was collected to detect the TNF‐α and IL‐6 according to the instructions of TNF alpha Mouse ProQuantum Immunoassay Kit (A43658, Thermo Fisher Scientific) and IL‐6 Mouse ProQuantum Immunoassay Kit (A43656, Thermo Fisher Scientific) though microplate reader (Invitrogen; Thermo Fisher Scientific, Inc.).
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