R plex human calprotectin antibody set

Manufactured by Mesoscale

The R-plex Human Calprotectin Antibody Set is a laboratory equipment product designed for the detection and quantification of calprotectin, a protein complex found in neutrophils and monocytes. The set includes capture and detection antibodies for use in immunoassay techniques to measure calprotectin levels in biological samples.

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Lab products found in correlation

Bio plex manager 6, by Bio-Rad (2 mentions) S plex human ifnα2a kit, by Mesoscale (2 mentions) Bio plex 200 system, by Bio-Rad (2 mentions) V plex proinflammatory panel 1 human kit, by Mesoscale (1 mentions) U plex human il 18 assay, by Mesoscale (1 mentions)

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R-plex

3 protocols using r plex human calprotectin antibody set

Multiplex Cytokine Profiling of Serum

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Serum samples were centrifuged for 15 min at 1000 × g, diluted 1:4, then monitored using the Bio-Plex ProTM Human Chemokine Panel 40-plex Assay (Bio-rad, ref: 171AK99MR2) according to the manufacturer’s instructions. 40-plex cytokines and chemokines provided are CCL1, CCL11, CCL13, CCL15, CCL17, CCL19, CCL2, CCL20, CCL21, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL3, CCL7, CCL8, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL5, CXCL6, CXCL8, CXCL9, GM-CSF, IFN-γ, IL-10, IL-16, IL-1β, IL-2, IL-4, IL-6, MIF, TNF- α. Acquisitions and analyses were performed on a Bio-Plex 200 system (Bio-rad) and a Bio-Plex Manager 6.1 Software (Bio-rad), respectively. Soluble Calprotectin (diluted 1:100) and IFN-α2a were analyzed using a R-plex Human Calprotectin Antibody Set (Meso Scale Discovery, ref: F21YB-3) and the ultrasensitive assay S-plex Human IFN-α2a kit (Meso Scale Discovery, ref: K151P3S-1), respectively, following manufacturer’s instructions. Acquisitions and analyses of soluble Calprotectin and IFN-α2a were performed on a MESO™ QuickPlex SQ120 reader and the MSD’s Discovery Workbench 4.0. Each serum sample was assayed twice with the average value taken as the final result.

Goubet A.G., Dubuisson A., Geraud A., Danlos F.X., Terrisse S., Silva C.A., Drubay D., Touri L., Picard M., Mazzenga M., Silvin A., Dunsmore G., Haddad Y., Pizzato E., Ly P., Flament C., Melenotte C., Solary E., Fontenay M., Garcia G., Balleyguier C., Lassau N., Maeurer M., Grajeda-Iglesias C., Nirmalathasan N., Aprahamian F., Durand S., Kepp O., Ferrere G., Thelemaque C., Lahmar I., Fahrner J.E., Meziani L., Ahmed-Belkacem A., Saïdani N., La Scola B., Raoult D., Gentile S., Cortaredona S., Ippolito G., Lelouvier B., Roulet A., Andre F., Barlesi F., Soria J.C., Pradon C., Gallois E., Pommeret F., Colomba E., Ginhoux F., Kazandjian S., Elkrief A., Routy B., Miyara M., Gorochov G., Deutsch E., Albiges L., Stoclin A., Gachot B., Florin A., Merad M., Scotte F., Assaad S., Kroemer G., Blay J.Y., Marabelle A., Griscelli F., Zitvogel L, & Derosa L. (2021). Prolonged SARS-CoV-2 RNA virus shedding and lymphopenia are hallmarks of COVID-19 in cancer patients with poor prognosis. Cell Death and Differentiation, 28(12), 3297-3315.

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Serum Cytokine and Calprotectin Profiling

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Serum samples were monitored using the V-plex Proinflammatory panel 1 Human kit (Meso Scale Discovery, ref: K15049D) according to the manufacturer’s instructions, for the measurement of IFNγ, IL1β, IL2, IL4, IL6, IL8, IL10, IL12p70, IL13, and TNFα. Soluble Calprotectin (diluted 1:100), IFNα2a and IL18 were analyzed using a R-plex Human Calprotectin Antibody Set (Meso Scale Discovery, ref: F21YB), the ultra-sensitive assay S-plex Human IFNa2a kit (Meso Scale Discovery, ref: K151P3S) and the U-plex Human IL18 assay (Meso Scale Discovery, ref: K151VJK), respectively, following manufacturer’s instructions. Acquisitions and analyses of soluble cytokines and calprotectin were performed on a MESO QuickPlex SQ120 reader and the MSD’s Discovery Workbench 4.0. Each serum sample was assayed twice with the average value taken as the result.

Danlos F.X., Grajeda-Iglesias C., Durand S., Sauvat A., Roumier M., Cantin D., Colomba E., Rohmer J., Pommeret F., Baciarello G., Willekens C., Vasse M., Griscelli F., Fahrner J.E., Goubet A.G., Dubuisson A., Derosa L., Nirmalathasan N., Bredel D., Mouraud S., Pradon C., Stoclin A., Rozenberg F., Duchemin J., Jourdi G., Ellouze S., Levavasseur F., Albigès L., Soria J.C., Barlesi F., Solary E., André F., Pène F., Ackerman F., Mouthon L., Zitvogel L., Marabelle A., Michot J.M., Fontenay M, & Kroemer G. (2021). Metabolomic analyses of COVID-19 patients unravel stage-dependent and prognostic biomarkers. Cell Death & Disease, 12(3), 258.

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Multiplex Plasma Cytokine Profiling

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Plasma samples (Table S6) were centrifuged for 15 min at 1,000 g, diluted 1:4, then monitored using the Bio-Plex Pro™ Human Chemokine Panel 40-plex Assay (Bio-rad, ref: 171AK99MR2) according to the manufacturer’s instructions. 40-plex cytokines and chemokines provided are: CCL1, CCL11, CCL13, CCL15, CCL17, CCL19, CCL2, CCL20, CCL21, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL3, CCL7, CCL8, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL16, CXCL2, CXCL5, CXCL6, CXCL8, CXCL9, GM-CSF, IFNα, IL-10, IL-16, IL-1b, IL-2, IL-4, IL-6, MIF, TNFa. Acquisitions and analyses were performed on a Bio-Plex 200 system (Bio-rad) and a Bio-Plex Manager 6.1 Software (Bio-rad), respectively. Soluble Calprotectin (diluted 1:100) and IFNα2a were analyzed using a R-plex Human Calprotectin Antibody Set (Meso Scale Discovery, ref: F21YB-3) and the ultra-sensitive assay S-plex Human IFNα2a kit (Meso Scale Discovery, ref: K151P3S-1), respectively, following manufacturer’s instructions. Acquisitions and analyses of soluble Calprotectin and IFNα were performed on a MESO QuickPlex SQ120 reader and the MSD’s Discovery Workbench 4.0. Each plasma sample was assayed twice with the average value taken as the final result. Data representation was performed with software R v3.3.3 using tidyverse, dplyr, ggplot2, ggpubr, pheatmap, corrplot or Hmisc packages.

Silvin A., Chapuis N., Dunsmore G., Goubet A.G., Dubuisson A., Derosa L., Almire C., Hénon C., Kosmider O., Droin N., Rameau P., Catelain C., Alfaro A., Dussiau C., Friedrich C., Sourdeau E., Marin N., Szwebel T.A., Cantin D., Mouthon L., Borderie D., Deloger M., Bredel D., Mouraud S., Drubay D., Andrieu M., Lhonneur A.S., Saada V., Stoclin A., Willekens C., Pommeret F., Griscelli F., Ng L.G., Zhang Z., Bost P., Amit I., Barlesi F., Marabelle A., Pène F., Gachot B., André F., Zitvogel L., Ginhoux F., Fontenay M, & Solary E. (2020). Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19. Cell, 182(6), 1401-1418.e18.

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