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4 well cell culture slides

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The 4-well cell culture slides are a laboratory equipment designed for the cultivation and observation of cells in vitro. These slides provide a convenient platform for researchers to grow and study cells in a controlled environment. Each slide features four separate wells, allowing for the simultaneous cultivation of multiple cell types or experimental conditions. The slides are made of high-quality materials suitable for cell culture applications and are designed to facilitate efficient cell growth and observation.

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Lab products found in correlation

Axio imager m1 microscope, by Zeiss (2 mentions) Phalloidin cruzfluor 594 conjugate, by Santa Cruz Biotechnology (1 mentions) Ab91654, by Abcam (1 mentions) Dako serum free blocking solution, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Cell culture slides Culture wells

2 protocols using 4 well cell culture slides

1

Visualization of Actin Cytoskeleton in SMCs

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F-actin cytoskeleton was determined using Phalloidin CruzFluor™ 594 Conjugate (Santa Cruz Technology, sc-363795) staining. In brief, SMCs were seeded in on 4-well cell culture slides (MatTek) and serum-starved for 2 days, and then stimulated with 5% FBS with or without SIK inhibitor HG-9–91–01 for 16 h. Cells were fixed with 4% PFA, permeabilized in 0.2% Triton X-100, and stained with Phalloidin CruzFluor™ 594 Conjugate. The nuclei were stained with DAPI. Images of F-actin cytoskeleton were captured with a Zeiss AxioImager M1 microscope and qualified by ImageJ software.
Cai Y., Wang X.L., Lu J., Lin X., Dong J, & Guzman R.J. (2021). Salt-inducible kinase 3 promotes vascular smooth muscle cell proliferation and arterial restenosis by regulating AKT and PKA-CREB signaling. Arteriosclerosis, thrombosis, and vascular biology, 41(9), 2431-2451.
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2

Immunofluorescent Staining of SMCs

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SMCs were seeded on 4-well cell culture slides (MatTek), fixed in 4% PFA, permeabilized in 0.1% Triton x-100/PBS, and blocked with Dako serum-free blocking solution (Dako, X090930). The immunofluorescent staining was conducted using the primary antibody, followed by incubation of secondary antibody Alexa Fluor 546 or 488 (ThermoFisher Scientific). The primary antibodies against BrdU (Bio-Rad, MCA2483T) and CRTC3 (Abcam, ab91654) were used. Nuclei were stained with DAPI. The staining was visualized with Zeiss AxioImager M1 microscope.
Cai Y., Wang X.L., Lu J., Lin X., Dong J, & Guzman R.J. (2021). Salt-inducible kinase 3 promotes vascular smooth muscle cell proliferation and arterial restenosis by regulating AKT and PKA-CREB signaling. Arteriosclerosis, thrombosis, and vascular biology, 41(9), 2431-2451.
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