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Elia celikey igg

Manufactured by Thermo Fisher Scientific

The EliA Celikey IgG is a laboratory equipment product designed for the detection and quantification of IgG antibodies against tissue transglutaminase, a specific marker for celiac disease. The product utilizes the enzyme-linked immunosorbent assay (ELISA) technology to provide accurate and reliable results.

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2 protocols using elia celikey igg

1

Serological Biomarkers for Celiac Disease

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Detection of IgA anti-TG2, IgA anti-GL, and Immunoglobulin G (IgG) anti-DGP in serum or plasma was performed using EliA Celikey IgA (positive result ≥ 7 U/mL), EliA Gliadin IgA (positive result ≥ 7 U/mL), and EliA GliadinDP IgG (positive result ≥ 10 U/mL), respectively, on Phadia250 (Thermo Fisher Scientific, Waltham, MA) as described by the manufacturer. In cases with total IgA levels below 0.07 g/L, detection of IgG anti-TG2 replaced IgA anti-TG2 (EliA Celikey IgG, Thermo Fisher Scientific). In order to distinguish results below the detection limit of an assay from missing data, the former were replaced with the detection limit divided by two.
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2

Evaluation of Celiac Disease Diagnostics

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The tTG IgA reference test was EliA Celikey IgA (Thermo Scientific, Phadia GmbH), a FEIA used widely by clinical laboratories. Total IgA was measured with an accredited in-house method of HUSLAB (Hospital District of Helsinki and Uusimaa, Laboratory Services, Finland). If the total IgA measurement was indicative of selective IgA deficiency, tTG IgG was measured by EliA Celikey IgG (Thermo Scientific, Phadia GmbH).
A commercial rapid lateral flow test (Celiac Disease Quick Test, Biohit) for anti-tTG antibodies performed according to the manufacturer’s instructions was used as an additional reference.
All statistical analyses were performed with R version 3.5.1.
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