Nigericin tlrl nig

Manufactured by InvivoGen

Sourced in United States

Nigericin is a polyether ionophore antibiotic isolated from Streptomyces hygroscopicus. It functions as a potassium ionophore, facilitating the exchange of potassium ions across cell membranes.

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Lab products found in correlation

Rabbit anti eif2α, by Cell Signaling Technology (1 mentions) Rabbit anti ire1, by Cell Signaling Technology (1 mentions) Atp a6419, by Merck Group (1 mentions) Rabbit anti p erk, by Cell Signaling Technology (1 mentions) Rabbit anti caspase 1 p10, by Santa Cruz Biotechnology (1 mentions) Rabbit anti asc, by Santa Cruz Biotechnology (1 mentions) Anti mouse il 1β, by R&D Systems (1 mentions) Txnip, by Santa Cruz Biotechnology (1 mentions) Mouse anti xbp1s, by BioLegend (1 mentions) Rabbit anti nf κb p65 d14e12, by Cell Signaling Technology (1 mentions) Rabbit anti caspase 1, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit antibody, by Jackson ImmunoResearch (1 mentions) Rabbit anti actin, by Merck Group (1 mentions) Z vad fmk, by Promega (1 mentions) Z devd fmk, by R&D Systems (1 mentions) Sb202190 ab120638, by Abcam (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Ab210070, by Abcam (1 mentions) Ab143839, by Abcam (1 mentions) Af 401 na, by R&D Systems (1 mentions)

3 protocols using nigericin tlrl nig

Molecular Mechanisms of Endoplasmic Reticulum Stress

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Primary antibodies used were as follows: mouse anti-XBP1s (Biolegend, 647502), rabbit anti-IRE1 (Cell Signaling, 3294), rabbit anti-PERK (Cell Signaling Technology, 3192), rabbit anti-eIF2α (Cell Signaling Technology, 5324), rabbit anti-p(S51)-eIF2α (Cell Signaling Technology, 3398), mouse anti-ATF6 (CosmoBio, BAM-73-500-EX), rabbit anti-NLRP3 D2P5E (Cell signaling technology, 13158), rabbit anti-NF-κB p65 (D14E12) (Cell signaling technology, 8242), mouse anti-IL-1β (R&D Systems, MAB601), rabbit anti-ASC (Santa Cruz, sc-22514-R), rabbit anti-caspase 1 (Santa Cruz, sc-622), rabbit anti-caspase-1 p10 (Santa Cruz, sc-515), TXNIP (Santa Cruz, sc-166234), and rabbit anti-Actin (Sigma, A2066). Secondary antibodies were horseradish peroxidase-tagged goat anti-mouse (Jackson Laboratories, 115-035-003) and goat anti-rabbit antibodies (Jackson Laboratories, 111-035-003). Tunicamycin (T7765), Phorbol 12-myristate 13-acetate (PMA) (P8139), LPS (L2630), and ATP (A6419) were purchased from Sigma-Aldrich while nigericin (tlrl-nig) was obtained from Invivogen. IRE1 inhibitor MKC8866 was provided by Fosun Orinove.

Talty A., Deegan S., Ljujic M., Mnich K., Naicker S.D., Quandt D., Zeng Q., Patterson J.B., Gorman A.M., Griffin M.D., Samali A, & Logue S.E. (2019). Inhibition of IRE1α RNase activity reduces NLRP3 inflammasome assembly and processing of pro-IL1β. Cell Death & Disease, 10(9), 622.

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Recombinant TGF-β3 for Cell Stimulation

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Recombinant human TGF‐β (TGF‐β3; #243‐B3‐010) was obtained from R&D Systems (Minneapolis, MN, USA). Nigericin (#tlrl‐nig) was purchased from InvivoGen (San Diego, CA, USA), and lipopolysaccharide (LPS) (#L4391) was from Sigma‐Aldrich (Merck, Darmstadt, Germany). Z‐VAD‐FMK (#G7232) was from Promega (Madison, WI, USA), and Z‐DEVD‐FMK (#FMK004) was from R&D Systems. SB202190 (#ab120638) was from Abcam (Cambridge, UK), and SB203580 (#tlrl‐sb20) was from InvivoGen. LY364947 was from Calbiochem (#616451; Merck).

Tamura Y., Morikawa M., Tanabe R., Miyazono K, & Koinuma D. (2021). Anti‐pyroptotic function of TGF‐β is suppressed by a synthetic dsRNA analogue in triple negative breast cancer cells. Molecular Oncology, 15(5), 1289-1307.

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Characterization of Pyroptosis Signaling

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The primary antibodies used were against IL-1β (AF-401-NA, R&D), caspase-1 (AG-20B-0042-C100, Adipogene), GSDMD (ab210070, Abcam), MLKL (generated in house (74 (link)), available from Millipore, MABC604, clone 3H1) and β-actin (A-1978, Sigma-Aldrich). Flow cytometric staining utilized the following antibodies from BioLegend; purified anti-mouse CD16/32 antibody (101302), APC-conjugated anti-Ly6G (127614), FITC-conjugated anti-F4/80 (123108), PE-conjugated anti-B220 (103208), APC-CY7-conjugated anti-CD3 (100222) and Brilliant Violet 421-conjugated anti-CD11b (101236). 123 count eBeads were purchased from Thermo scientific (01-1234-42). Ultrapure LPS (tlrl-smlps) and nigericin (tlrl-nig) were purchased from InvivoGen. MSU was generated in house, as previously described(63 (link), 75 (link)). Imject™ Alum Adjuvant (77161, thermo scientific), Z-VAD-fmk (187389-52-2, Santa Cruz Biotechnology), CA-074-Me (sc-214647, Santa Cruz Biotechnology), necrosulfonamide (ab143839, Abcam), potassium chloride (P954, Sigma-Aldrich) and glycine (G7126; Sigma-Aldrich) were purchased as indicated.

Rashidi M., Simpson D.S., Hempel A., Frank D., Petrie E., Vince A., Feltham R., Murphy J., Chatfield S.M., Salvesen G.S., Murphy J.M., Wicks I.P, & Vince J.E. (2019). The pyroptotic cell death effector Gasdermin D is activated by gout-associated uric acid crystals but is dispensable for cell death and IL-1β release. Journal of immunology (Baltimore, Md. : 1950), 203(3), 736-748.

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