The intravitreal injections were performed as described [51 (link)] with the following modifications. Immediately after the laser coagulation, 9.0 pg or 9.0 ng of ISRIB (Cayman Chemical, Ann Arbor, MI, USA) in 2 µL [10 nM or 10 µM, dissolved in phosphate-buffered saline (PBS) containing 0.1% dimethyl sulfoxide (DMSO)] was injected into the vitreous cavity with a Hamilton glass syringe (701N; Hamilton Co., Reno, NV, USA) fitted with a 34-G nanopass needle (Terumo, Tokyo, Japan). Then, 5 µL of 0.01% levofloxacin ophthalmic solution (Santen Pharmaceuticals Co., Ltd., Osaka, Japan) was applied topically.
34 g nanopass needle
Manufactured by Terumo
Sourced in United States
The 34-G nanopass needle is a high-precision medical device designed for intradermal, subcutaneous, and intramuscular injections. It features a small-gauge needle with a narrow diameter for minimally invasive procedures. The needle is constructed with advanced materials to ensure durability and precision during use.
Automatically generated - may contain errors
2 protocols using 34 g nanopass needle
1
Intravitreal ISRIB Injection Protocol
2
Intravitreal PDGF Injection in Mice
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Mice were anesthetized with isoflurane (Merck Hoei Ltd., Osaka, Japan), and 2 lL 10 or 100 ng recombinant murine PDGF-BB (Peprotech, Rocky Hill, NJ, USA) or 100 ng recombinant murine PDGF-AA (Peprotech) was injected intravitreally into the left eyes 2 hours before light exposure. Control mice were injected with the same volume of 0.01 M phosphate-buffered saline (PBS) into the left eyes. All solutions were injected into the vitreous with a 10-lL Hamilton glass syringe (701N; Hamilton Co., Reno, NV, USA) fitted with a 34-G nanopass needle (Terumo, Tokyo, Japan). After the intravitreal injection, 5 lL 0.01% levofloxacin ophthalmic solution (Santen Pharmaceuticals Co., Ltd.) was applied topically.
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