Static light scattering data were obtained using a Superdex 75 Increase 10/300 GL column (GE Healthcare) coupled with a miniDAWN (3-angle) light scattering detector (Wyatt Technology) and an Optilab T-rEX refractive index detector (Wyatt Technology). The column was equilibrated with 20 mM HEPES, pH 7.0 and 150 mM NaCl. Then, 100 μl of AcrIE4-F7 (150 μM) was loaded onto the column at a flow rate of 0.5 ml/min at 25°C. The results were analyzed using the ASTRA 8 software (Wyatt Technology).
Minidawn 3 angle light scattering detector
Manufactured by Wyatt Technology
The MiniDAWN (3-angle) is a light scattering detector used to measure the molar mass and size of macromolecules and nanoparticles. It utilizes three fixed-angle detectors to determine the absolute molar mass and radius of gyration of samples in solution.
Automatically generated - may contain errors
Lab products found in correlation
Superdex 75 increase 10 300 gl column, by GE Healthcare (1 mentions)
Optilab t rex refractive index detector, by Wyatt Technology (1 mentions)
Astra 8, by Wyatt Technology (1 mentions)
Superdex 200 10 300 column, by GE Healthcare (1 mentions)
Optilab rex refractometer, by Wyatt Technology (1 mentions)
Astra software, by Wyatt Technology (1 mentions)
2 protocols using minidawn 3 angle light scattering detector
1
Static Light Scattering Analysis of AcrIE4-F7
2
MALLS-SEC Analysis of MtTMEM175
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3C-protease cleaved MtTMEM175 protein was purified as described above except that the peak fraction after size exclusion chromatography was diluted to 35 μM (1 mg/ml) before subjecting it to MALLS-SEC using a Superdex 200 10/300 column (GE healthcare) with an Agilent LC-1100 system coupled to an Optilab rEX refractometer (Wyatt Technology) and a miniDAWN 3-angle light-scattering detector (Wyatt Technology). The SEC buffer contained 150 mM KCl, 10 mM Hepes-NaOH and 0.03% DDM at pH 7.6 at RT. Data was analyzed with ASTRA software (Wyatt Technology).
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