13c3 labelled acrylamide

Avocado (Persea Americana L. var. Hass) seeds were supplied by Terravocado S.A. (Medellín-Colombia). The fresh potato tubers (Solanum tuberosum L., var. R12 Diacol Capiro) were supplied by the Instituto Colombiano Agropecuario (ICA, Colombia). The commercial fresh potatoes and palm oil (Oleollano, Villavicencio-Meta, Colombia; Lot L24082020F2TC YP) were purchased from a local market (Medellín, Colombia). The commercial French fries were purchased in a street food establishment (Medellín, Colombia). Acrylamide standard (99%), potassium hexacyanoferrate (II) trihydrate (98%, Carrez-I), and zinc acetate dehydrate (> 99%, Carrez-II) were obtained from Sigma (St. Louis, MO, USA). ¹³C₃-labelled acrylamide (99% isotopic purity) was obtained from Cambridge Isotope Laboratories (Andover, MA, USA). Formic acid (98%), methanol (99.5%), sulfuric acid (97%), phenol, anhydrous glucose and hexane were from Panreac (Barcelona, Spain). Sodium bisulfite was from Merck (Darmstadt, Germany) and glycerol from Bell Chem International S.A.S (Antioquia, Colombia). Deionized water was obtained from a milli-Q integral 5 water purification system (Millipore, Billerica, MA, USA). All other chemicals, solvents and reagents were of analytical grade.

Acrylamide standard (99%), potassium hexacyanoferrate (II) trihydrate (98%, Carrez-I), and zinc acetate dehydrate (>99%, Carrez-II) were obtained from Sigma (St. Louis, MO, USA). ¹³C₃-labelled acrylamide (99% isotopic purity) was obtained from Cambridge Isotope Laboratories (Andover, MA, USA). Formic acid (98%), methanol (99.5%) and hexane were from Panreac (Barcelona, Spain). Deionized water was obtained from a Milli-Q Integral 5 water purification system (Millipore, Billerica, MA, USA). All other chemicals, solvents and reagents were of analytical grade. Reversed-phase Oasis-HLB cartridges (30 mg, 1 mL) were from Waters (Mildford, MA, USA). Syringe filter units (0.45 µm and 0.20 µm, cellulose) were purchased from Análisis Vínicos (Tomelloso, Ciudad Real, Spain).

Potassium hexacyanoferrate (II) trihydrate (98%, Carrez-I) and zinc acetate dehydrate (>99%, Carrez-II) were obtained from Sigma (St. Louis, USA). 13C3-labelled acrylamide (99% isotopic purity) was obtained from Cambridge Isotope Laboratories (Andover, MA, USA). HMF was purchased from Sigma (St. Louis, MO, USA). Formic acid (98%) was obtained from Panreac (Barcelona, Spain). Deionized water was obtained from a Milli-Q Integral 5 water purification system (Millipore, Billerica, MA, USA). Reversed-phase Oasis-HLB cartridges (30 mg, 1 mL) were provided by Waters (Milford, MA, USA). High performance liquid chromatography (HPLC)-grade methanol was obtained from Merck (Darmstadt, Germany). All other chemicals, solvents and reagents were of analytical grade.