Disodium hydrogen phosphate na2hpo4

Acetic acid, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2, 4, 6 tripyridyl-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), gallic acid, linoleic acid, and hydrochloric acid (HCl) were purchased from Sigma Aldrich (St. Louis, MO, USA). Collagenase from Clostridium histolyticum, N-[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala (FALGPA), elastase from porcine pancreas, N-succinyl-Ala-Ala-Ala-p-nitroanilide (AAAVPN), disodium hydrogen phosphate (Na₂HPO₄), potassium dihydrogen phosphate (KH₂PO₄), potassium persulfate (K₂S₂O₈), potassium chloride (KCl), sodium carbonate (Na₂CO₃), sodium chloride (NaCl), ferric chloride (FeCl₃), ferrous sulfate (FeSO₄), ferrous chloride (FeCl₂), and ammonium thiocyanate (NH₄SCN) were purchased from Fisher Chemicals (Loughborough, UK). Bovine serum albumin was purchased from Merck (Darmstadt, Germany). Tricine and tris base were purchased from Bio-Rad Laboratories (Richmond, CA, USA). Sodium acetate trihydrate (CH₃COONa·3H2O), sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), and ethanol were analytical grade and purchased from RCI Labscan Co., Ltd. (Bangkok, Thailand).

Waste cotton clothes were kindly provided by Renaissance (Montreal, QC Canada), and soybean protein isolate (SPI) was provided by Cargill (Minneapolis, MN, USA). Hydrogen peroxide (H₂O₂), sodium dihydrogen phosphate (NaH₂PO₄), and disodium hydrogen phosphate (Na₂HPO₄) were purchased from Fisher Scientific (Ottawa, ON, Canada) and used as received. Reagent alcohol (95%) was obtained from the RICCA Chemical company (Arlington, VA, USA). Sulfuric acid (H₂SO₄), and sodium periodate (NaIO₄), sodium chloride (NaCl), ethylene glycol, sodium chlorite (NaClO₂), sodium hypochlorite (NaClO), sodium hydroxide (NaOH), glycerol, and (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO) were purchased from Sigma-Aldrich (Oakville, ON, Canada) and used without further treatment.