miR-23a mimics (miR-23a), control mimics (miR-control), siRNA against GAS5 (si-GAS5), siRNA control (si-control), pcDNA-GAS5, and pcDNA empty control (pcDNA-control) were purchased from GenePharma (Shanghai, P.R. China). Cultured A549 and H157 cells were transfected with miR-23a, si-GAS5, pcDNA-GAS5, pcDNA-GAS5 + miR-23a, or their corresponding control at approximately 60%–70% confluence using Lipofectamine 2000 (Invitrogen). Transfection efficiency was checked by quantitative real-time polymerase chain reaction (qRT-PCR). Subsequent experiments were performed 48 h after transfection.
Mei Y., Si J., Wang Y., Huang Z., Zhu H., Feng S., Wu X, & Wu L. (2017). Long Noncoding RNA GAS5 Suppresses Tumorigenesis by Inhibiting miR-23a Expression in Non-Small Cell Lung Cancer. Oncology Research, 25(6), 1027-1037.
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