Dulbecco's modified Eagle's medium (DMEM), Roswell Park Memorial Institute (RPMI 1640) medium, fetal bovine serum (FBS), 4′,6‐diamidino‐2‐phenylindole (DAPI) stain solution, and 0.25% trypsin‐EDTA solution was purchased from Sangon Biotech. Penicillin–streptomycin and Phorbol 12‐myristate 13‐acetate (PMA) were purchased from Solarbio Science & Technology Co., Ltd. Ammonium fluoride and ethylene glycol (Sinopharm chemical reagent). The cell counting kit‐8 (CCK‐8) solution and Matrigel (BD Biosciences). Lactic acid assay Kit (Colorimetric method) and Glucose assay Kit (O‐toluidine method) were purchased from Lengton Biotechnology. Anti‐Glut1, anti‐HK2, anti‐CD86, anti‐CD206, donkey anti‐rabbit secondary antibodies, anti‐AMPK‐α, and AMPK inhibitors were purchased from Abcam. A medical‐grade Ti rod was purchased from Baoti.
Cell counting kit 8 cck 8 solution
Manufactured by BD
Sourced in United States
The Cell Counting Kit-8 (CCK-8) solution is a colorimetric assay used to determine the number of viable cells in a sample. It contains a water-soluble tetrazolium salt that is reduced by cellular dehydrogenase enzymes, resulting in the formation of an orange-colored formazan dye. The intensity of the color is directly proportional to the number of living cells, which can be measured using a spectrophotometer.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Solarbio (1 mentions)
Matrigel, by BD (1 mentions)
Donkey anti rabbit secondary antibody, by Abcam (1 mentions)
Anti cd206, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Sangon (1 mentions)
Rpmi 1640 medium, by Sangon (1 mentions)
Anti hk2, by Abcam (1 mentions)
Dulbecco s modified eagle s medium, by Sangon (1 mentions)
Anti cd86, by Abcam (1 mentions)
Anti ampkα, by Abcam (1 mentions)
Anti glut1, by Abcam (1 mentions)
Phorbol 12 myristate 13 acetate pma, by Solarbio (1 mentions)
Prism 6, by GraphPad (1 mentions)
2 protocols using cell counting kit 8 cck 8 solution
1
Macrophage Polarization and Metabolic Profiling
2
Cell Proliferation Assay with CCK-8
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Transfected cells were plated in 96-well plates (1,000 cells/well) and incubated with 10 µL of Cell Counting Kit 8 (CCK-8) solution (BD, Franklin Lakes, NJ, USA) at 37 ℃ for 2 hours, and then the absorption was measured at 450 nm. Proliferation rates were detected at days 1, 2, 3, 4, and 5 after transfection. Proliferation rates were calculated using GraphPad Prism 6.0 software (GraphPad Software, San Diego, CA, USA).
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