Digital micrograph 1

For TEM analysis, the strains were cultivated under anoxic conditions in FSM at 24°C for 48 h, fixed in formaldehyde (1.8%), adsorbed onto carbon-coated copper grids (F200-CU carbon support film, 200 mesh; Electron Microscopy Sciences, Hatfield, UK), and washed three times with double-distilled water (ddH₂O). TEM was performed on a JEM-2100 instrument (JEOL Ltd., Tokyo, Japan) at 80 kV. Images were captured with a Gatan model 782 ES500W Erlangshen charge-coupled device camera (Gatan Inc., Pleasanton, CA, USA) with the software Digital Micrograph 1.80.70 (Gatan Inc.). For data analysis and measurements, the software ImageJ Fiji V1.50c (93 (link)) was used.

For TEM analysis, strains (wild type, Δtrans-at-pks, and P_mamDC45-trans-at-pks) cultivated in 6-well plates (Sarstedt, Nümbrecht, Germany) under microoxic conditions at 24°C for 48 h, fixed in formaldehyde (1.8%), adsorbed onto carbon-coated copper grids (F200-CU carbon support film, 200 mesh; Electron Microscopy Sciences, Hatfield, UK), and washed three times with double-distilled water (ddH₂O). TEM was performed on a JEM-2100 instrument (JEOL Ltd., Tokyo, Japan) with an accelerating voltage of 80 kV. Images were captured with a Gatan model 782 ES500W Erlangshen charge-coupled-device (CCD) camera (Gatan Inc., Pleasanton, CA) with the software Digital Micrograph 1.80.70 (Gatan Inc.). For data analysis and measurements, the software ImageJ Fiji V1.50c (56 (link)) was used.